Western Blot (WB)
(POLR3B Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).)
Rabbit anti-Mouse POLR3B Polyclonal Antibody
POLR3B, NT (POLR3B, DNA-directed RNA polymerase III subunit RPC2, C128, DNA-directed RNA polymerase III 127.6kD polypeptide, DNA-directed RNA polymerase III subunit B)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
POLR3B; Polyclonal Antibody; NT (POLR3B; DNA-directed RNA polymerase III subunit RPC2; C128; DNA-directed RNA polymerase III 127.6kD polypeptide; DNA-directed RNA polymerase III subunit B); Anti -POLR3B; anti-POLR3B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-POLR3B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
POLR3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-359 amino acids from the N-terminal region of human POLR3B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(POLR3B Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).)
Western Blot (WB)
(POLR3B Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).)
Related Product Information for anti-POLR3B antibody
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.
Product Categories/Family for anti-POLR3B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
POLR3B
Similar Products
Product Notes
The POLR3B (Catalog #AAA6012503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLR3B, NT (POLR3B, DNA-directed RNA polymerase III subunit RPC2, C128, DNA-directed RNA polymerase III 127.6kD polypeptide, DNA-directed RNA polymerase III subunit B) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's POLR3B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the POLR3B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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