Rabbit anti-Mouse POLR3B Polyclonal Antibody | anti-POLR3B antibody

POLR3B, NT (POLR3B, DNA-directed RNA polymerase III subunit RPC2, C128, DNA-directed RNA polymerase III 127.6kD polypeptide, DNA-directed RNA polymerase III subunit B) (PE)

Cat. Num. AAA6335784

• Gene Names: POLR3B; C128; HLD8; RPC2; INMAP
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR3B; Polyclonal Antibody; NT (POLR3B; DNA-directed RNA polymerase III subunit RPC2; C128; DNA-directed RNA polymerase III 127.6kD polypeptide; DNA-directed RNA polymerase III subunit B) (PE); DNA-directed RNA polymerase III subunit B; anti-POLR3B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1133

• Applicable Applications for anti-POLR3B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLR3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-359 amino acids from the N-terminal region of human POLR3B.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR3B Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).)

Related Product Information for anti-POLR3B antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Product Categories/Family for anti-POLR3B antibody

Antibodies; Enzymes; Polymerase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Ablasser, A., et al. Nat. Immunol. 10(10):1065-1072(2009). Shen, E., et al. Exp. Cell Res. 315(7):1100-1116(2009). Yee, N.S., et al. PLoS Biol. 5 (11), E312 (2007): Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 238908503
• NCBI GeneID: 55703
• NCBI Accession #: NP_060552.4
• NCBI GenBank Nucleotide #: NP_060552.4
• UniProt Accession #: Q9NW08
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC2 isoform 1
• NCBI Official Synonym Full Names: RNA polymerase III subunit B
• NCBI Official Symbol: POLR3B
• NCBI Official Synonym Symbols: C128; HLD8; RPC2; INMAP
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC2
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC2
• UniProt Gene Name: POLR3B
• UniProt Synonym Gene Names: RNA polymerase III subunit C2
• UniProt Entry Name: RPC2_HUMAN

NCBI Description

This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]