Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC1 antibody (Ser421/423))
Rabbit HDAC1 Polyclonal Antibody | anti-HDAC1 antibody
HDAC1 Antibody (Ser421/423)
Gene Names
HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
HDAC1; Polyclonal Antibody; HDAC1 Antibody (Ser421/423); Rabbit Polyclonal HDAC1 Antibody (Ser421/423); Polyclonal HDAC1 antibody; Anti-HDAC1 antibody; DKFZp686H12203 antibody; GON-10 antibody; HD1 antibody; HDAC1 antibody; Histone deacetylase 1 antibody; reduced potassium dependency yeast homolog-like 1 antibody; RPD3 antibody; RPD3L1 antibody; anti-HDAC1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
HDAC1 antibody (Ser421/423) detects endogenous levels of HDAC1 only when phosphorylated at serine 421/423
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
482
Applicable Applications for anti-HDAC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:4000
IHC: 1:50-1:100
ELISA: 1:4000
Immunogen
HDAC1 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421/423 (E-F-SP-D- SP -E-E) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC1 antibody (Ser421/423))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC1 antibody (Ser421/423))
Western Blot (WB)
(Western blot analysis of extracts from HELA cells. Left:Using HDAC1 (Phospho-Ser421/423) antibody. Right: Using the same antibody preincubated with synthesized peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HELA cells. Left:Using HDAC1 (Phospho-Ser421/423) antibody. Right: Using the same antibody preincubated with synthesized peptide.)
Related Product Information for anti-HDAC1 antibody
HDAC1 is a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Product Categories/Family for anti-HDAC1 antibody
NCBI and Uniprot Product Information
NCBI Description
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Research Articles on HDAC1
Similar Products
Product Notes
The HDAC1 (Catalog #AAA5313780) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HDAC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:4000. Researchers should empirically determine the suitability of the HDAC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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