Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cell, Primary antibody: 161632(POLA1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
Rabbit anti-Human POLA1 Polyclonal Antibody | anti-POLA1 antibody
POLA1 Rabbit pAb
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cell, Primary antibody: 161632(POLA1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cell, Primary antibody: 161632(POLA1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]
Uniprot Description
POLA: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Belongs to the DNA polymerase type-B family.
Protein type: DNA replication; DNA repair, damage; Nuclear envelope; EC 2.7.7.7; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: Xp22.1-p21.3
Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; nuclear matrix; cytoplasm; nucleolus; nuclear envelope; chromatin; nucleus
Molecular Function: protein binding; DNA binding; DNA primase activity; protein heterodimerization activity; 4 iron, 4 sulfur cluster binding; metal ion binding; nucleoside binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin binding; protein kinase binding
Biological Process: DNA replication initiation; viral reproduction; DNA replication, synthesis of RNA primer; DNA strand elongation during DNA replication; leading strand elongation; DNA repair; lagging strand elongation; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; DNA synthesis during DNA repair; cell proliferation; telomere maintenance via recombination; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
Disease: N Syndrome
Research Articles on POLA1
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Product Notes
The POLA1 pola1 (Catalog #AAA4754799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLA1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the POLA1 pola1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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