Rabbit anti-Human, Mouse POH1 Polyclonal Antibody | anti-PSMD14 antibody

POH1 (Rpn11)

Cat. Num. AAA618098

• Gene Names: PSMD14; PAD1; POH1; RPN11
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: POH1; Polyclonal Antibody; POH1 (Rpn11); Anti -POH1 (Rpn11); anti-PSMD14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes the human and mouse POH1 proteins at ~35kD.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-PSMD14 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1-3ug/ml; Immunoprecipitation (Mouse): 7ug/IP reaction
• Immunogen: Synthetic peptide derived from C-terminal region of POH1 (26S proteosome-associated pad1 homolog/ non-ATPase subunit also known as Rpn11 in yeast.
• Positive Control: HeLa and NIH3T3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PSMD14 antibody

The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.

Product Categories/Family for anti-PSMD14 antibody

Antibodies; Abs to Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 42490917
• NCBI GeneID: 10213
• UniProt Accession #: O00487; O00176; B3KNW2
• Molecular Weight: 34,577 Da
• NCBI Official Full Name: Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• NCBI Official Symbol: PSMD14
• NCBI Official Synonym Symbols: PAD1; POH1; RPN11
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 14
• UniProt Gene Name: PSMD14
• UniProt Synonym Gene Names: POH1
• UniProt Entry Name: PSDE_HUMAN

NCBI Description

This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]

Uniprot Description

PSMD14: a component of the 26S proteasome. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Protein type: EC 3.4.19.-; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: proteasome complex; nucleoplasm; cytosol; nucleus

Molecular Function: metallopeptidase activity; metal ion binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis