Rabbit anti-Human PSMD14 Polyclonal Antibody | anti-PSMD14 antibody

PSMD14, CT (PSMD14, POH1, 26S proteasome non-ATPase regulatory subunit 14, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1) (Azide free) (HRP)

Cat. Num. AAA6338264

• Gene Names: PSMD14; PAD1; POH1; RPN11
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMD14; Polyclonal Antibody; CT (PSMD14; POH1; 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit RPN11; 26S proteasome-associated PAD1 homolog 1) (Azide free) (HRP); 26S proteasome-associated PAD1 homolog 1; anti-PSMD14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMD14.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PSMD14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 258-287 in the C-terminal region of human PSMD14, UniProt Accession #O00487. Species sequence homology: mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSMD14 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PSMD14 antibody detected the PSMD14 protein (arrow).)

Related Product Information for anti-PSMD14 antibody

PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).

Product Categories/Family for anti-PSMD14 antibody

Antibodies; Proteasomes

References

Byrne, A., et al. Exp. Cell Res. 316(2):258-271(2010). Ma, Z., et al. Pediatr Int 51(5):732-735(2009). Cooper, E.M., et al. EMBO J. 28(6):621-631(2009). Koulich, E., et al. Mol. Biol. Cell 19(3):1072-1082(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5031981
• NCBI GeneID: 10213
• NCBI Accession #: NP_005796.1
• NCBI GenBank Nucleotide #: NM_005805.5
• UniProt Accession #: O00487; O00176; B3KNW2
• Molecular Weight: 34,577 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 14
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• NCBI Official Symbol: PSMD14
• NCBI Official Synonym Symbols: PAD1; POH1; RPN11
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 14
• UniProt Gene Name: PSMD14
• UniProt Synonym Gene Names: POH1
• UniProt Entry Name: PSDE_HUMAN

Uniprot Description

PSMD14: a component of the 26S proteasome. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Protein type: EC 3.4.19.-; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: proteasome complex; nucleoplasm; cytosol; nucleus

Molecular Function: metallopeptidase activity; metal ion binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis