Rabbit anti-Human PSMD14 Polyclonal Antibody | anti-PSMD14 antibody

Anti-PSMD14 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104030

• Gene Names: PSMD14; PAD1; POH1; RPN11
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMD14; Polyclonal Antibody; Anti-PSMD14 Antibody; Rabbit Polyclonal; PSMD14 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) 26S subunit; non-ATPase; 14; Anti-PAD1 Antibody; Anti-POH1 Antibody; Anti-RPN11 Antibody; anti-PSMD14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMD14
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMD14 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PSMD14
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PSMD14, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMD14 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa)

Related Product Information for anti-PSMD14 antibody

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein within the 19S complex (19S cap; PA700) is responsible for substrate deubiquitination during proteasomal degradation. PSMD14 is a 19S-proteasome-associated deubiquitinating enzyme that facilitates protein degradation by the 20S proteasome core particle. Accumulating evidence indicates that PSMD14 has emerged as a critical oncogenic factor by promoting tumor growth. PSMD14 may serve as a potential prognostic marker and therapeutic target for lung adenocarcinoma (LUAD) patients. The deubiquitinase PSMD14 acts as a positive regulator for the initiation of the BMP6 signaling pathway through deubiquitinating K48-linked ALK2 type I receptor ubiquitination mediated by Smurf1 E3 ligase, resulting in increased stability of the ALK2.

References

Zhang L, et al. (2020) Upregulation of deubiquitinase psmd14 in lung adenocarcinoma (luad) and its prognostic significance. J Cancer 11 (10): 2962-2971.Seo D, et al. (2019) The deubiquitinating enzyme psmd14 facilitates tumor growth and chemoresistance through stabilizing the alk2 receptor in the initiation of bmp6 signaling pathway. EBioMedicine 49 55-71.Byrne A, et al. (2010) Knockdown of human deubiquitinase psmd14 induces cell cycle arrest and senescence. Exp Cell Res 316 (2): 258-271.

NCBI and Uniprot Product Information

• NCBI GI #: 42490917
• NCBI GeneID: 10213
• UniProt Accession #: O00487; O00176; B3KNW2
• Molecular Weight: 34,577 Da
• NCBI Official Full Name: Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
• NCBI Official Symbol: PSMD14
• NCBI Official Synonym Symbols: PAD1; POH1; RPN11
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 14
• UniProt Gene Name: PSMD14
• UniProt Synonym Gene Names: POH1
• UniProt Entry Name: PSDE_HUMAN

NCBI Description

This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]

Uniprot Description

PSMD14: a component of the 26S proteasome. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

Protein type: EC 3.4.19.-; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: proteasome complex; nucleoplasm; cytosol; nucleus

Molecular Function: metallopeptidase activity; metal ion binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; double-strand break repair via nonhomologous end joining; double-strand break repair via homologous recombination; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis