Western Blot (WB)
(Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).)
Rabbit anti-Human ENTPD3 Polyclonal Antibody | anti-ENTPD3 antibody
ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6)
Gene Names
ENTPD3; HB6; CD39L3; NTPDase-3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ENTPD3; Polyclonal Antibody; CT (ENTPD3; CD39L3; Ectonucleoside triphosphate diphosphohydrolase 3; CD39 antigen-like 3; Ecto-ATP diphosphohydrolase 3; Ecto-apyrase 3; HB6); Anti -ENTPD3; anti-ENTPD3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-ENTPD3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
ENTPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-531 amino acids from the C-terminal region of human ENTPD3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in K562 and HL-60 cell line lysates (35ug/lane) using MBS646154. This demonstrates that MBS646154 detected the ENTPD3 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646154. Peroxidase-conjugated secondary antibody and DAB staining were used to visualize the antigen.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS646154. Peroxidase-conjugated secondary antibody and DAB staining were used to visualize the antigen.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat anti-rabbit secondary antibody was used.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat anti-rabbit secondary antibody was used.)
Related Product Information for anti-ENTPD3 antibody
ENTPD3 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases.
Product Categories/Family for anti-ENTPD3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,105 Da
NCBI Official Full Name
ENTPD3 protein
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 3
NCBI Official Symbol
ENTPD3
NCBI Official Synonym Symbols
HB6; CD39L3; NTPDase-3
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 3; NTPDase 3; CD39-like 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 3
Protein Family
UniProt Gene Name
ENTPD3
UniProt Synonym Gene Names
CD39L3; NTPDase 3; Ecto-ATPDase 3
UniProt Entry Name
ENTP3_HUMAN
NCBI Description
ENTPD3 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Has a threefold preference for the hydrolysis of ATP over ADP.
Catalytic activity: A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.
Cofactor: Ca2+ or Mg2+.
Subcellular location: Membrane; Multi-pass membrane protein
Potential.
Tissue specificity: Expressed in adult brain, pancreas, spleen and prostate. Moderate or low expression is seen in most tissues. Not expressed in liver and peripheral blood leukocytes.
Sequence similarities: Belongs to the GDA1/CD39 NTPase family.
Research Articles on ENTPD3
Similar Products
Product Notes
The ENTPD3 entpd3 (Catalog #AAA646154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENTPD3, CT (ENTPD3, CD39L3, Ectonucleoside triphosphate diphosphohydrolase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-apyrase 3, HB6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENTPD3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the ENTPD3 entpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENTPD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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