Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human PNCK Polyclonal Antibody | anti-PNCK antibody
PNCK, CT (PNCK, Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:50-100
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.)
Western Blot (WB)
(Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.)
NCBI and Uniprot Product Information
NCBI Description
PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1
By similarity.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Activated by Ca2+/calmodulin
By similarity.
Subcellular location: Cytoplasm
By similarity. Nucleus
By similarity.
Post-translational modification: Phosphorylated by CAMKK1
Probable.
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.
Research Articles on PNCK
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Product Notes
The PNCK pnck (Catalog #AAA647272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PNCK, CT (PNCK, Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNCK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the PNCK pnck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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