SDS-PAGE
(Lane 1: 5 uL MW Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.)
Mouse IgG (Fc) Immunoglobulin | IgG immunoglobulin
Mouse IgG (Fc)
Applications
User optimized
Purity
Single arc observed by IEP.
Synonyms
Mouse IgG (Fc); Mouse Immunoglobulin G (Fc); IgG immunoglobulin
Host
Mouse
Purity/Purification
Single arc observed by IEP.
Form/Format
Sterile filtered liquid, supplied in 20mM PBS buffer, 150mM Soidum Chloride, pH 7.2 with 0.01% Sodium Azide.
Concentration
1.0 mg/ml by OD280nm (varies by lot)
Applicable Applications for IgG immunoglobulin
User optimized
Grade
Highly Purified
Source
Mouse Serum delipidated, fractionated, ion-exchange chromatography & papain difestion.
Biohazard Information
Use Normal Laboratory Techniques when handling this material
Biological Significance
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fc regions of IgGs bear a highly conserved N-glycosylation site. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and alpha-2,6-linked sialic acid residues.
Preparation and Storage
Ships ambient or refrigerated. Upon receipt store at 4 degree C under sterile conditions. For extended storage aliquot and freeze at -20 degree C. Prepare fresh working dilutions daily.
SDS-PAGE
(Lane 1: 5 uL MW Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.)
SDS-PAGE
(Lane 1: 5 uL MW Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.)
Related Product Information for IgG immunoglobulin
Purified Mouse IgG (Fc)
Product Categories/Family for IgG immunoglobulin
Similar Products
Product Notes
The IgG (Catalog #AAA537224) is an Immunoglobulin produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mouse IgG (Fc) can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse IgG (Fc), Immunoglobulin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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