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Western Blot (WB) (Western blot analysis of PMPCB Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). PMPCB (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse PMPCB Polyclonal Antibody | anti-PMPCB antibody

PMPCB, NT (PMPCB, MPPB, Mitochondrial-processing peptidase subunit beta, Beta-MPP, P-52)

Gene Names
PMPCB; MPPB; P-52; MPP11; MPPP52; Beta-MPP
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
PMPCB; Polyclonal Antibody; NT (PMPCB; MPPB; Mitochondrial-processing peptidase subunit beta; Beta-MPP; P-52); Anti -PMPCB; anti-PMPCB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-PMPCB antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
PMPCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 25-55 from the N-terminal region of human PMPCB.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PMPCB Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). PMPCB (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PMPCB Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). PMPCB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PMPCB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMPCB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PMPCB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMPCB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PMPCB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (PMPCB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PMPCB antibody
PMPCB is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.
Product Categories/Family for anti-PMPCB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
54,366 Da
NCBI Official Full Name
PMPCB protein, partial
NCBI Official Synonym Full Names
peptidase (mitochondrial processing) beta
NCBI Official Symbol
PMPCB
NCBI Official Synonym Symbols
MPPB; P-52; MPP11; MPPP52; Beta-MPP
NCBI Protein Information
mitochondrial-processing peptidase subunit beta; mitochondrial processing peptidase beta subunit
UniProt Protein Name
Mitochondrial-processing peptidase subunit beta
UniProt Gene Name
PMPCB
UniProt Synonym Gene Names
MPPB
UniProt Entry Name
MPPB_HUMAN

NCBI Description

This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex. [provided by RefSeq, Jul 2008]

Uniprot Description

PMPCB: Cleaves presequences (transit peptides) from mitochondrial protein precursors. Belongs to the peptidase M16 family.

Protein type: Mitochondrial; EC 3.4.24.64; Protease

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function: metal ion binding; metalloendopeptidase activity

Biological Process: cellular protein metabolic process; proteolysis; protein targeting to mitochondrion

Research Articles on PMPCB

Similar Products

Product Notes

The PMPCB pmpcb (Catalog #AAA6002332) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PMPCB, NT (PMPCB, MPPB, Mitochondrial-processing peptidase subunit beta, Beta-MPP, P-52) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PMPCB can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the PMPCB pmpcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMPCB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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