Sheep Breast Cancer Susceptibility Protein 2 ELISA Kit | BRCA2 elisa kit

Sheep Breast Cancer Susceptibility Protein 2 ELISA Kit

Cat. Num. AAA735290

• Gene Names: BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; BROVCA2
• Reactivity: Sheep
• Synonyms: Breast Cancer Susceptibility Protein 2; Sheep Breast Cancer Susceptibility Protein 2 ELISA Kit; BRCA2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This BRCA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep BRCA2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BRCA2 elisa kit

Principle of the assay: BRCA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BRCA2 antibody and an BRCA2-HRP conjugate. The assay sample and buffer are incubated together with BRCA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BRCA2 concentration since BRCA2 from samples and BRCA2-HRP conjugate compete for the anti-BRCA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BRCA2 from the sample, fewer sites are left to bind BRCA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BRCA2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BRCA2 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 1161384
• NCBI GeneID: 675
• Molecular Weight: 384,225 Da
• NCBI Official Full Name: BRCA2
• NCBI Official Synonym Full Names: breast cancer 2, early onset
• NCBI Official Symbol: BRCA2
• NCBI Official Synonym Symbols: FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; BROVCA2
• NCBI Protein Information: breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset
• UniProt Protein Name: Breast cancer type 2 susceptibility protein
• Protein Family: Breast cancer type 2 susceptibility protein
• UniProt Gene Name: BRCA2
• UniProt Synonym Gene Names: FACD; FANCD1
• UniProt Entry Name: BRCA2_HUMAN

NCBI Description

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

Uniprot Description

BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.

Protein type: Nuclear receptor co-regulator; DNA repair, damage; Tumor suppressor

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule

Molecular Function: gamma-tubulin binding; protein binding; H4 histone acetyltransferase activity; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding

Biological Process: positive regulation of transcription, DNA-dependent; cell aging; cytokinesis; positive regulation of mitotic cell cycle; DNA repair; oocyte maturation; regulation of cytokinesis; inner cell mass cell proliferation; negative regulation of mammary gland epithelial cell proliferation; double-strand break repair via homologous recombination; response to UV-C; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; replication fork protection; spermatogenesis; hemopoiesis; brain development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; centrosome duplication; female gonad development; response to X-ray

Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Medulloblastoma; Prostate Cancer; Breast Cancer; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1