Rabbit anti-Human, Mouse PLK3 Polyclonal Antibody | anti-PLK3 antibody

PLK3, CT (PLK3, CNK, FNK, PRK, Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, Proliferation-related kinase) (FITC)

Cat. Num. AAA6335007

• Gene Names: PLK3; CNK; FNK; PRK
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PLK3; Polyclonal Antibody; CT (PLK3; CNK; FNK; PRK; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; Proliferation-related kinase) (FITC); Proliferation-related kinase; anti-PLK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PLK3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 617~646 amino acids from the C-terminal region of human PLK3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CNK Pab in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B). CNK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-PLK3 antibody

CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle. It may also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. This membrane-associated protein binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. This protein exhibits a short half-live. In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Cytokine and cellular adhesion trigger CNK induction. CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.

Product Categories/Family for anti-PLK3 antibody

Antibodies; Protein Kinases

References

Holtrich, U., et al., Oncogene 19(42):4832-4839 (2000). Ouyang, B., et al., Oncogene 18(44):6029-6036 (1999). Ouyang, B., et al., J. Biol. Chem. 272(45):28646-28651 (1997). Li, B., et al., J. Biol. Chem. 271(32):19402-19408 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 41872374
• NCBI GeneID: 1263
• NCBI Accession #: NP_004064.2
• NCBI GenBank Nucleotide #: NM_004073.2
• UniProt Accession #: Q9H4B4; Q15767; Q5JR99; Q96CV1
• Molecular Weight: 71,629 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK3
• NCBI Official Synonym Full Names: polo-like kinase 3
• NCBI Official Symbol: PLK3
• NCBI Official Synonym Symbols: CNK; FNK; PRK
• NCBI Protein Information: serine/threonine-protein kinase PLK3; PLK-3; FGF-inducible kinase; cytokine-inducible kinase; proliferation-related kinase; cytokine-inducible serine/threonine-protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase PLK3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK3
• UniProt Synonym Gene Names: CNK; FNK; PRK; PLK-3
• UniProt Entry Name: PLK3_HUMAN

NCBI Description

Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.

Protein type: Nucleolus; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: centrosome; Golgi stack; cell soma; cytoplasm; dendrite; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; ATP binding

Biological Process: apoptosis; mitotic cell cycle checkpoint; negative regulation of transcription from RNA polymerase II promoter; endomitotic cell cycle; response to osmotic stress; protein amino acid phosphorylation; regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; response to reactive oxygen species; response to radiation; protein kinase B signaling cascade; G2/M transition of mitotic cell cycle; regulation of cell division; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle