Rabbit anti-Human POLDIP3 Polyclonal Antibody | anti-POLDIP3 antibody

POLDIP3, NT (POLDIP3, KIAA1649, PDIP46, Polymerase delta-interacting protein 3, 46kD DNA polymerase delta interaction protein, S6K1 Aly/REF-like target) (PE)

Cat. Num. AAA6335597

• Gene Names: POLDIP3; SKAR; PDIP46
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLDIP3; Polyclonal Antibody; NT (POLDIP3; KIAA1649; PDIP46; Polymerase delta-interacting protein 3; 46kD DNA polymerase delta interaction protein; S6K1 Aly/REF-like target) (PE); S6K1 Aly/REF-like target; anti-POLDIP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-POLDIP3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLDIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human POLDIP3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLDIP3 Antibody (N-term) western blot analysis in Jurkat,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the POLDIP3 Antibody antibody detected the POLDIP3 Antibody protein (arrow).)

Related Product Information for anti-POLDIP3 antibody

This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq].

Product Categories/Family for anti-POLDIP3 antibody

Antibodies; KIAA; orf

References

Ma, X.M., et al. Cell 133(2):303-313(2008). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Smyk, A., et al. FEBS J. 273(20):4728-4741(2006). Andersen, J.S., et al. Nature 433(7021):77-83(2005). Richardson, C.J., et al. Curr. Biol. 14(17):1540-1549(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 29837655
• NCBI GeneID: 84271
• NCBI Accession #: NP_115687.2
• NCBI GenBank Nucleotide #: NM_032311.3
• UniProt Accession #: Q9BY77; Q009A7; Q5H972; Q6PGN6; Q7Z6Z0; Q9NSP5; Q9NSP6; A8K6F8; A8K6V9
• Molecular Weight: 46,089 Da
• NCBI Official Full Name: polymerase delta-interacting protein 3 isoform 1
• NCBI Official Synonym Full Names: polymerase (DNA-directed), delta interacting protein 3
• NCBI Official Symbol: POLDIP3
• NCBI Official Synonym Symbols: SKAR; PDIP46
• NCBI Protein Information: polymerase delta-interacting protein 3; p46; RNA-binding protein P46; S6K1 Aly/REF-like target; polymerase delta interacting protein 46; 46 kDa DNA polymerase delta interaction protein
• UniProt Protein Name: Polymerase delta-interacting protein 3
• Protein Family: Polymerase delta-interacting protein
• UniProt Gene Name: POLDIP3
• UniProt Synonym Gene Names: KIAA1649; PDIP46; p46; SKAR
• UniProt Entry Name: PDIP3_HUMAN

NCBI Description

This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SKAR: Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: protein binding; nucleotide binding

Biological Process: poly(A)+ mRNA export from nucleus; positive regulation of translation