Rabbit RIG-1 Polyclonal Antibody | anti-RIG-1 antibody

RIG-1 (Retinoic Acid Inducible Gene 1 Protein, RIG-I, Probable ATP-Dependent RNA Helicase DDX58, Dead Box Protein 58, DDX58) (Biotin)

Cat. Num. AAA6497831

• Gene Names: DHX58; LGP2; RLR-3; D11LGP2; D11lgp2e
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography from whole serum.
• Synonyms: RIG-1; Polyclonal Antibody; RIG-1 (Retinoic Acid Inducible Gene 1 Protein; RIG-I; Probable ATP-Dependent RNA Helicase DDX58; Dead Box Protein 58; DDX58) (Biotin); anti-RIG-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RIG-I. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by affinity chromatography from whole serum.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-RIG-1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate, pH 6, is recommended.; Applications are based on unconjugated antibody.
• Immunogen: Human GST-tagged RIG-1 protein.
• Conjugate: Biotin
• Positive Control: Human heart
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of C2C12 whole cell lysate using at 0.5ug/ml (lane A), 1ug/ml (lane B) and 2ug/ml (lane C).)

Immunohistochemistry (IHC)

(Immunohistochemistry staining of paraffin-embedded human heart tissue using.)

Related Product Information for anti-RIG-1 antibody

RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.

Product Categories/Family for anti-RIG-1 antibody

Antibodies; Enzymes

References

1. Akira S, et al (2006) Pathogen recognition and innate immunity 124:783-801. 2. Yoneyama M et al (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol.; 5:730-737. 3. Sharma S, et al (2003) Triggering the interferon antiviral response through an IKK-related pathway Science 300:1148-51. 4. Alexopoulou L, et al (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3 Nature 413:732-8.

NCBI and Uniprot Product Information

• NCBI GI #: 119581202
• NCBI GeneID: 79132
• UniProt Accession #: Q96C10; O95786
• Molecular Weight: 76,613 Da
• NCBI Official Full Name: likely ortholog of mouse D11lgp2, isoform CRA_b
• NCBI Official Synonym Full Names: DEXH (Asp-Glu-X-His) box polypeptide 58
• NCBI Official Symbol: DHX58
• NCBI Official Synonym Symbols: LGP2; RLR-3; D11LGP2; D11lgp2e
• NCBI Protein Information: probable ATP-dependent RNA helicase DHX58; RLR; RNA helicase LGP2; RIG-I-like receptor 3; protein D11Lgp2 homolog; RIG-I-like receptor LGP2; ortholog of mouse D11lgp2; probable ATP-dependent helicase LGP2
• UniProt Protein Name: Probable ATP-dependent RNA helicase DHX58
• UniProt Gene Name: DHX58
• UniProt Synonym Gene Names: D11LGP2E; LGP2; RLR-3; RLR
• UniProt Entry Name: DHX58_HUMAN

Uniprot Description

DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily.

Protein type: Helicase; EC 3.6.4.13; Inhibitor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm

Molecular Function: protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: viral reproduction; negative regulation of innate immune response; metabolic process; response to virus; positive regulation of interferon type I production; innate immune response; defense response to virus; negative regulation of interferon type I production; regulation of innate immune response