Rabbit anti-Human PLK1 / PLK-1 Polyclonal Antibody | anti-PLK1 antibody

Rabbit Polyclonal to Human PLK1 / PLK-1

Cat. Num. AAA244400

• Gene Names: PLK1; PLK; STPK13
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PLK1 / PLK-1; Polyclonal Antibody; Rabbit Polyclonal to Human PLK1 / PLK-1; Anti-PLK1 / PLK-1 Antibody IHC-plus; PLK1; Polo like kinase; Polo-like kinase (Drosophila); Polo-like kinase 1; STPK13; Polo (Drosophia)-like kinase; PLK; PLK-1; Human PLK1; anti-PLK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human PLK1. Predicted cross-reactivity based on amino acid sequence homology: mouse (95%), rat (95%), bovine (95%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 603

• Applicable Applications for anti-PLK1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:500 - 1:3000)
• Target Species: Human
• Immunogen Description: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 161 and 440 of Human PLK1.
• Immunogen Type: Recombinant protein
• Immunogen: PLK1 / PLK-1 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 161 and 440 of Human PLK1.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PLK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PLK1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PLK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Sample(30 ug whole cell lysate). A: Raji. 7.5% SDS PAGE. PLK1 antibody diluted at 1:1000.)

Related Product Information for anti-PLK1 antibody

PLK, a Polo type protein kinase, has been shown to function in mitosis. The mRNA is absent or expressed at very low levels during the G1 phase, begins to reaccumulate during the S phase, and reaches maximal levels during the G2/M phase. PLK1 may participate in targeting MPF to the nucleus during prophase and in regulating mitotic spindle function. These functions, in addition to its expression in proliferating cells, suggest that PLK1 has a role in cancer progression.

Product Categories/Family for anti-PLK1 antibody

Family: Protein Kinase; Subfamily: Polo

NCBI and Uniprot Product Information

• NCBI GI #: 21359873
• NCBI GeneID: 5347
• NCBI Accession #: NP_005021.2
• NCBI GenBank Nucleotide #: NM_005030.3
• UniProt Accession #: P53350; Q15153; Q99746
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo-like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1; PLK-1; polo like kinase; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; cell cycle regulated protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13
• UniProt Entry Name: PLK1_HUMAN

NCBI Description

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.

Protein type: EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Other group; PLK family

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; kinetochore; spindle pole; centrosome; spindle microtubule; cytoplasm; nucleolus; spindle; midbody; spindle midzone; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of proteolysis; regulation of cell cycle; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; anaphase-promoting complex activation during mitotic cell cycle; cytokinesis; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to antibiotic; establishment of protein localization; cytokinesis after mitosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; centrosome organization and biogenesis; positive regulation of ubiquitin-protein ligase activity; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; mitosis; mitotic sister chromatid segregation; protein destabilization; organelle organization and biogenesis; regulation of mitotic metaphase/anaphase transition; negative regulation of cyclin-dependent protein kinase activity; cell proliferation; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; polar body extrusion after meiotic divisions; mitotic cell cycle; G2/M transition DNA damage checkpoint; mitotic metaphase/anaphase transition; microtubule bundle formation; negative regulation of apoptosis