Human Hyaluronan Synthase 1 (HAS1) RTU ELISA Kit | HAS1 rtu elisa kit

Human Hyaluronan Synthase 1 (HAS1) ELISA Kit

Cat. Num. AAA459794

• Gene Names: HAS1; HAS
• Reactivity: Human
• Synonyms: Hyaluronan Synthase 1 (HAS1); Human Hyaluronan Synthase 1 (HAS1) ELISA Kit; HuHAS1; HA synthase 1; Hyaluronate synthase 1; Hyaluronic acid synthase 1; HAS1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 578

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HAS1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HAS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HAS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HAS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 167466171
• NCBI GeneID: 3036
• NCBI Accession #: NP_001514.2
• NCBI GenBank Nucleotide #: NM_001523.3
• UniProt Accession #: Q92839; Q14470; Q9NS49
• Molecular Weight: 64,832 Da
• NCBI Official Full Name: hyaluronan synthase 1 isoform 1
• NCBI Official Synonym Full Names: hyaluronan synthase 1
• NCBI Official Symbol: HAS1
• NCBI Official Synonym Symbols: HAS
• NCBI Protein Information: hyaluronan synthase 1
• UniProt Protein Name: Hyaluronan synthase 1
• Protein Family: Hyaluronan synthase
• UniProt Gene Name: HAS1
• UniProt Synonym Gene Names: HAS; HA synthase 1; HuHAS1

NCBI Description

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate ().