Mouse anti-Human Cytokeratin 8 Monoclonal Antibody | anti-KRT8 antibody

Cytokeratin 8 Mouse Monoclonal

Cat. Num. AAA768455

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Protein A+G purification
• Synonyms: Cytokeratin 8; Monoclonal Antibody; Cytokeratin 8 Mouse Monoclonal; anti-KRT8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Purity/Purification: >=95% as determined by SDS-PAGE; Protein A+G purification
• Form/Format: Liquid
• Sequence Length: 482

• Applicable Applications for anti-KRT8 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Keratin 8
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach tissue slide using MBS768455 (KRT8 Antibody) at dilution of 1:50)

Western Blot (WB)

(A549 cells were subjected to SDS PAGE followed by western blot with MBS768455 (KRT8 Antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 181573
• NCBI GeneID: 3856
• Molecular Weight: 56,608 Da
• NCBI Official Full Name: cytokeratin 8
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: costamere; cytoplasm; dystrophin-associated glycoprotein complex; intercellular junction; intermediate filament; keratin filament; nuclear matrix; nucleoplasm; nucleus; sarcolemma; Z disc

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: response to hydrostatic pressure; response to other organism; sarcomere organization; tumor necrosis factor-mediated signaling pathway; viral reproduction

Disease: Cirrhosis, Familial