Rabbit anti-Human PLIN2 / ADFP / Adipophilin Polyclonal Antibody | anti-PLIN2 antibody

Rabbit Polyclonal to Human PLIN2 / ADFP / Adipophilin

Cat. Num. AAA244946

• Gene Names: PLIN2; ADFP; ADRP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: PLIN2 / ADFP / Adipophilin; Polyclonal Antibody; Rabbit Polyclonal to Human PLIN2 / ADFP / Adipophilin; Anti-PLIN2 / ADFP / Adipophilin Antibody IHC-plus; PLIN2; Adipophilin; ADFP; ADRP; Perilipin 2; Perilipin-2; Human PLIN2; anti-PLIN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human ADFP. Predicted cross-reactivity based on amino acid sequence homology: mouse (82%), bovine (86%), pig (87%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 437

• Applicable Applications for anti-PLIN2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:500 - 1:3000)
• Target Species: Human
• Immunogen Description: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 28 and 281 of Human ADFP.
• Immunogen Type: Recombinant protein
• Immunogen: PLIN2 / ADFP / Adipophilin antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 28 and 281 of Human ADFP.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Adipophilin antibody IHC of human liver, lipid droplets. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Adipophilin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: Hep G2. 10% SDS PAGE. ADFP antibody diluted at 1:1000.)

Related Product Information for anti-PLIN2 antibody

Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 34577059
• NCBI GeneID: 123
• NCBI Accession #: NP_001113.2
• NCBI GenBank Nucleotide #: NM_001122.3
• UniProt Accession #: Q99541; Q9BSC3
• Molecular Weight: 48,075 Da
• NCBI Official Full Name: perilipin-2
• NCBI Official Synonym Full Names: perilipin 2
• NCBI Official Symbol: PLIN2
• NCBI Official Synonym Symbols: ADFP; ADRP
• NCBI Protein Information: perilipin-2; adipophilin; adipose differentiation-related protein
• UniProt Protein Name: Perilipin-2
• Protein Family: Perilipin
• UniProt Gene Name: PLIN2
• UniProt Synonym Gene Names: ADFP; ADRP
• UniProt Entry Name: PLIN2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane; lipid particle; cytosol; nucleus

Biological Process: response to drug; long-chain fatty acid transport; sequestering of lipid; cellular lipid metabolic process; response to organic cyclic substance