Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6008984. This demonstrates that MBS6008984 detected the ENPEP protein (arrow).)
Rabbit anti-Human ENPEP Polyclonal Antibody | anti-ENPEP antibody
ENPEP, CT (ENPEP, Glutamyl aminopeptidase, Aminopeptidase A, Differentiation antigen gp160, CD249) (APC)
Gene Names
ENPEP; APA; CD249; gp160
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ENPEP; Polyclonal Antibody; CT (ENPEP; Glutamyl aminopeptidase; Aminopeptidase A; Differentiation antigen gp160; CD249) (APC); CD249; anti-ENPEP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ENPEP.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ENPEP antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 899-930 in the C-terminal region of human ENPEP, UniProt Accession #NP_001968.3; Q07075.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6008984. This demonstrates that MBS6008984 detected the ENPEP protein (arrow).)
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS6008984. This demonstrates that MBS6008984 detected the ENPEP protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6008984 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008984 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6008984 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008984 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of NCI-H460 cells using MBS6008984 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of NCI-H460 cells using MBS6008984 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ENPEP antibody
ENPEP appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
Product Categories/Family for anti-ENPEP antibody
References
1. Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010). 2. Rose, J. Phd, et al. Mol. Med. (2010). In press 3. Perez, I., et al. Head Neck 31(10):1335-1340(2009). 4. Sevalle, J., et al. J. Neurochem. 109(1):248-256(2009). 5. Teranishi, J., et al. Prostate 68(15):1666-1673(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,244 Da
NCBI Official Full Name
glutamyl aminopeptidase
NCBI Official Synonym Full Names
glutamyl aminopeptidase
NCBI Official Symbol
ENPEP
NCBI Official Synonym Symbols
APA; CD249; gp160
NCBI Protein Information
glutamyl aminopeptidase
UniProt Protein Name
Glutamyl aminopeptidase
UniProt Gene Name
ENPEP
UniProt Synonym Gene Names
EAP; AP-A
Uniprot Description
Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Research Articles on ENPEP
Similar Products
Product Notes
The ENPEP enpep (Catalog #AAA6295645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENPEP, CT (ENPEP, Glutamyl aminopeptidase, Aminopeptidase A, Differentiation antigen gp160, CD249) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPEP can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENPEP enpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPEP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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