Rabbit anti-Human ADFP Polyclonal Antibody | anti-ADFP antibody

ADFP, ID (PLIN2, ADFP, Perilipin-2, Adipophilin, Adipose differentiation-related protein) (FITC)

Cat. Num. AAA6271731

• Gene Names: PLIN2; ADFP; ADRP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ADFP; Polyclonal Antibody; ID (PLIN2; Perilipin-2; Adipophilin; Adipose differentiation-related protein) (FITC); PLIN2; Adipose differentiation-related protein; anti-ADFP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADFP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADFP antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 186-214 in the central region of human ADFP, UniProt Accession #Q99541.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6001422. ADFP (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded breast carcinoma using MBS6001422 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001422 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells using MBS6001422 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ADFP antibody

Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.

Product Categories/Family for anti-ADFP antibody

Antibodies; Obesity Proteins

References

1. Kimmel, A.R., et al. J. Lipid Res. 51(3):468-471(2010). 2. Kotokorpi, P., et al. Mol. Pharmacol. 77(1):79-86(2010). 3. Minnaard, R., et al. J. Clin. Endocrinol. Metab. 94(10):4077-4085(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 34577059
• NCBI GeneID: 123
• NCBI Accession #: NP_001113.2
• NCBI GenBank Nucleotide #: NM_001122.3
• UniProt Accession #: Q99541; Q9BSC3
• Molecular Weight: 48,075 Da
• NCBI Official Full Name: perilipin-2
• NCBI Official Synonym Full Names: perilipin 2
• NCBI Official Symbol: PLIN2
• NCBI Official Synonym Symbols: ADFP; ADRP
• NCBI Protein Information: perilipin-2; adipophilin; adipose differentiation-related protein
• UniProt Protein Name: Perilipin-2
• UniProt Gene Name: PLIN2
• UniProt Synonym Gene Names: ADFP; ADRP
• UniProt Entry Name: PLIN2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane; lipid particle; nucleus; cytosol

Biological Process: long-chain fatty acid transport; response to drug; sequestering of lipid; cellular lipid metabolic process; response to organic cyclic substance