Mouse Plexin A1 Monoclonal Antibody | anti-PLXNA1 antibody

Plexin A1 (Sema Domain) M535 Antibody

Cat. Num. AAA474524

• Gene Names: PLXNA1; NOV; NOVP; PLXN1; PLEXIN-A1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
• Synonyms: Plexin A1; Monoclonal Antibody; Plexin A1 (Sema Domain) M535 Antibody; Plexin A1 (Sema Domain); Plexin A1 mAb; anti-PLXNA1 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M535
• Specificity: Clone M535 was affinity purified using Plexin A1 (Sema Domain) peptide (without carrier). The antibody detects a 220kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal mouse brain, and detects a recombinant mouse Plexin A1 protein containing the extracellular region.
• Form/Format: Mouse monoclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-PLXNA1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000; ICC: 1:100; IHC: 1:100; IP: 1:50; WB: 1:500
• Immunogen: Clone M535 was generated from a Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blots showing mouse recombinant Plexin-A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin-A1 (PP1301) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (PM5351) at 1:250 (lane 3) and 1:1000 (lane 4).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (PM5351). The antibody was detected using goat anti-mouse DyLight 594.)

Related Product Information for anti-PLXNA1 antibody

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

References

Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
Tamagnone, L. et al. (1999) Cell 99:71.
Kolodkin, A.L. et al. (1993) Cell 75:1389.

NCBI and Uniprot Product Information

• NCBI GI #: 262118282
• NCBI GeneID: 5361
• NCBI Accession #: NP_115618.3
• NCBI GenBank Nucleotide #: NM_032242.3
• UniProt Accession #: Q9UIW2
• Molecular Weight: 211,067 Da
• NCBI Official Full Name: plexin-A1
• NCBI Official Synonym Full Names: plexin A1
• NCBI Official Symbol: PLXNA1
• NCBI Official Synonym Symbols: NOV; NOVP; PLXN1; PLEXIN-A1
• NCBI Protein Information: plexin-A1; plexin 1; semaphorin receptor NOV
• UniProt Protein Name: Plexin-A1
• Protein Family: Plexin
• UniProt Gene Name: PLXNA1
• UniProt Synonym Gene Names: NOV; PLXN1

Uniprot Description

PLXNA1: Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down- stream signaling events in the cytoplasm. Interacts directly with NRP1 and NRP2. Interacts with FARP2, RND1 and KDR/VEGFR2. Binding of SEMA3A leads to dissociation of FARP2. Detected in fetal brain, lung, liver and kidney. Belongs to the plexin family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: extracellular exosome; integral component of membrane; plasma membrane

Molecular Function: receptor activity; semaphorin receptor activity

Biological Process: branchiomotor neuron axon guidance; multicellular organism development; regulation of axon extension involved in axon guidance; regulation of smooth muscle cell migration; semaphorin-plexin signaling pathway