Elafin Recombinant Protein | PI3 recombinant protein

Elafin Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121115

• Gene Names: PI3; ESI; WAP3; SKALP; WFDC14; cementoin
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Elafin; Elafin Protein; Human; Recombinant (His Tag); Human PI3/Elafin/WFDC14 Protein (His Tag); cementoin Protein; ESI Protein; MGC13613 Protein; SKALP Protein; WAP3 Protein; WFDC14 Protein; peptidase inhibitor 3; PI3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln117

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PI3 (NP_002629.1)(Met 1-Gln 117) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PI3 recombinant protein

Background: Elafin, also known as Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, PI3, WAP3 and WFDC14, is a secreted protein that contains one WAP domain. Elafin/PI3 consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain at the N-terminus serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. The serine anti-protease Elafin/PI3 is expressed by monocytes, alveolar macrophages, neutrophils, and at mucosal surfaces and possesses antimicrobial activity. It is also known to reduce lipopolysaccharide-induced neutrophil influx into murine alveoli as well as to abrogate lipopolysaccharide-induced production of matrix metalloprotease 9, macrophage inhibitory protein 2, and tumor necrosis factor-alpha by as-yet unidentified mechanisms. Elafin/PI3 is a neutrophil serine protease inhibitor expressed in lung and displaying anti-inflammatory and anti-bacterial properties. Elafin/PI3 is a neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Elafin/PI3 will regulate proteolytic enzymes during menstruation and will contribute to the innate defense against uterine infection.

Product Categories/Family for PI3 recombinant protein

Serine Proteases and Regulators

References

Skolnik E.Y., et al.,(1991), Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. Cell 65:83-90.Antonetti D.A., et al., (1996), Insulin receptor substrate 1 binds two novel splice variants of the regulatory subunit of phosphatidylinositol 3-kinase in muscle and brain.Mol. Cell. Biol. 16:2195-2203.Deloukas P., et al.,(2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.

NCBI and Uniprot Product Information

• NCBI GI #: 4505787
• NCBI GeneID: 5266
• NCBI Accession #: NP_002629.1
• NCBI GenBank Nucleotide #: NM_002638.3
• UniProt Accession #: P19957; Q6FG74; E1P618
• Molecular Weight: 12,270 Da
• NCBI Official Full Name: elafin preproprotein
• NCBI Official Synonym Full Names: peptidase inhibitor 3
• NCBI Official Symbol: PI3
• NCBI Official Synonym Symbols: ESI; WAP3; SKALP; WFDC14; cementoin
• NCBI Protein Information: elafin
• UniProt Protein Name: Elafin
• Protein Family: Elafin
• UniProt Gene Name: PI3
• UniProt Synonym Gene Names: WAP3; WFDC14; ESI; PI-3; SKALP
• UniProt Entry Name: ELAF_HUMAN

Uniprot Description

elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Protein type: Extracellular matrix; Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix

Molecular Function: endopeptidase inhibitor activity