Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3) Recombinant Protein | PAFAH1B3 recombinant protein

Recombinant Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)

Cat. Num. AAA2018710

• Gene Names: Pafah1b3; Pafahg; mus[g]
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3); Recombinant Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3); PAFAH1B3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSGEGENPAS KPTPVQDVQG DGRWMSLHHR FVADSKDKEP EVVFIGDSLV QLMHQCEIWR ELFSPLHALN FGIGGDSTQH VLWRLENGEL EHIRPKIVVV WVGTNNHSHT AEQVTGGIKA IVQLVNKLQP QARVVVLGLL PRGQHPNPLR EKNRQVNELV RAALAGYPRA HFLDADPGFV HSDGTISHHD MYDYLHLSRLGYTPVCRALH SLLLRLLAQD QGQGIPLPET AS
• Sequence Length: 232

• Applicable Applications for PAFAH1B3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Ser232 (Accession # Q61205) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6679201
• NCBI GeneID: 18476
• NCBI Accession #: NP_032802.1
• NCBI GenBank Nucleotide #: NM_008776.2
• UniProt Accession #: Q61205
• Molecular Weight: 29.7kDa
• NCBI Official Full Name: platelet-activating factor acetylhydrolase IB subunit gamma
• NCBI Official Synonym Full Names: platelet-activating factor acetylhydrolase, isoform 1b, subunit 3
• NCBI Official Symbol: Pafah1b3
• NCBI Official Synonym Symbols: Pafahg; mus[g]
• NCBI Protein Information: platelet-activating factor acetylhydrolase IB subunit gamma; PAF-AH gamma; PAFAH subunit gamma; PAF-AH subunit gamma; PAF-AH 29 kDa subunit; PAF acetylhydrolase 29 kDa subunit; platelet-activating factor acetylhydrolase, isoform 1b, alpha1 subunit
• UniProt Protein Name: Platelet-activating factor acetylhydrolase IB subunit gamma
• Protein Family: Platelet-activating factor acetylhydrolase
• UniProt Gene Name: Pafah1b3
• UniProt Synonym Gene Names: Pafahg; PAF-AH 29 kDa subunit
• UniProt Entry Name: PA1B3_MOUSE

Uniprot Description

PAFAH1B3: Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain. Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Protein type: Lipid Metabolism - ether lipid; Lipase; EC 3.1.1.47

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; hydrolase activity; platelet-activating factor acetyltransferase activity; protein heterodimerization activity; 1-alkyl-2-acetylglycerophosphocholine esterase activity

Biological Process: spermatogenesis; lipid metabolic process; brain development; lipid catabolic process