Goat anti-Human PLA2R1, Phospholipase A2 Receptor 1 Polyclonal Antibody | anti-PLA2R1 antibody

PLA2R1, Phospholipase A2 Receptor 1

Cat. Num. AAA626986

• Gene Names: PLA2R1; PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: PLA2R1; Phospholipase A2 Receptor 1; Polyclonal Antibody; Anti -PLA2R1; anti-PLA2R1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLA2R1. Species sequence homology: canine, bovine.
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PLA2R1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:64,000; Western Blot: 1-3ug/ml ~140kD band observed in Human Ileum lysates (calculated MW of 153kD according to NP_001007268.1).
• Immunogen: Synthetic peptide corresponding to C-EKKKDTPKQHGT, from the Internal region of human PLA2R1 (NP_031392.3; NP_001007268.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS626986 (1ug/ml) staining of Human Ileum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-PLA2R1 antibody

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 [PubMed 7721806]).

Product Categories/Family for anti-PLA2R1 antibody

Antibodies; Abs to Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 219519166
• NCBI GeneID: 22925
• UniProt Accession #: Q13018; Q13019; Q15095; Q53R45; Q53RR7; B2RTU9; D3DPB1
• Molecular Weight: 168,600 Da
• NCBI Official Full Name: PLA2R1 protein
• NCBI Official Synonym Full Names: phospholipase A2 receptor 1, 180kDa
• NCBI Official Symbol: PLA2R1
• NCBI Official Synonym Symbols: PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• NCBI Protein Information: secretory phospholipase A2 receptor; M-type receptor; OTTHUMP00000205042; OTTHUMP00000205043; C-type lectin domain family 13 member C; 180 kDa secretory phospholipase A2 receptor
• UniProt Protein Name: Secretory phospholipase A2 receptor
• Protein Family: Secretory phospholipase A2 receptor
• UniProt Gene Name: PLA2R1
• UniProt Synonym Gene Names: CLEC13C
• UniProt Entry Name: PLA2R_HUMAN

NCBI Description

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.

Uniprot Description

PLA2R1: Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2- IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2- IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow- derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q23-q24

Cellular Component: cell surface; integral to plasma membrane; integral to membrane; extracellular region; plasma membrane; receptor complex

Molecular Function: phospholipase binding; receptor activity; carbohydrate binding

Biological Process: receptor-mediated endocytosis; cytokine production; endocytosis; positive regulation of DNA damage response, signal transduction by p53 class mediator