Goat anti-Human PLA2R1 Polyclonal Antibody | anti-PLA2R1 antibody

PLA2R1, Phospholipase A2 Receptor 1 (HRP)

Cat. Num. AAA6493276

• Gene Names: PLA2R1; PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: PLA2R1; Polyclonal Antibody; Phospholipase A2 Receptor 1 (HRP); anti-PLA2R1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLA2R1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PLA2R1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to C-EKKKDTPKQHGT, from the internal region of human PLA2R1 (NP_031392.3; NP_001007268.1; NP_001182570.1). Species sequence homology: canine and porcine
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Testing Data

(P4074-11 (1ug/ml) staining of Human Ileum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-PLA2R1 antibody

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 [PubMed 7721806]).

Product Categories/Family for anti-PLA2R1 antibody

Antibodies; Enzymes; Phospholipase

References

1. Silliman CC, Moore EE, Zallen G, Gonzalez R, Johnson JL, Elzi DJ, Meng X, Hanasaki K, Ishizaki J, Arita H, Ao L, England KM, Banerjee A.Presence of the M-type sPLA(2) receptor on neutrophils and its role in elastase release and adhesion.American journal of physiology. Cell physiology 2002 Oct 283 (4): C1102-13.

NCBI and Uniprot Product Information

• NCBI GI #: 55953104
• NCBI GeneID: 22925
• NCBI Accession #: NP_031392.3; NP_001007268.1; NP_001182570.1
• NCBI GenBank Nucleotide #: NM_007366.4
• Molecular Weight: 152,805 Da
• NCBI Official Full Name: secretory phospholipase A2 receptor isoform 1
• NCBI Official Synonym Full Names: phospholipase A2 receptor 1
• NCBI Official Symbol: PLA2R1
• NCBI Official Synonym Symbols: PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R
• NCBI Protein Information: secretory phospholipase A2 receptor
• Protein Family: Secretory phospholipase A2 receptor

NCBI Description

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]