Western Blot (WB)
(Western blot analysis of extracts from HepG2, using PLA2G4F Antibody. Lane 1 was treated with the blocking peptide.)
Rabbit anti-Human, Mouse PLA2G4F Polyclonal Antibody | anti-PLA2G4F antibody
PLA2G4F Antibody
Gene Names
PLA2G4F; PLA2G4FZ
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PLA2G4F; Polyclonal Antibody; PLA2G4F Antibody; cPLA2-zeta; Cytosolic phospholipase A2 zeta; DKFZp666G192; DKFZp781B229; PA24F_HUMAN; Phospholipase A2 group IVF; Pla2g4f; PLA2G4FZ; anti-PLA2G4F antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PLA2G4F antibody detects endogenous levels of total PLA2G4F
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
849
Applicable Applications for anti-PLA2G4F antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human PLA2G4F
Subcellular Location
Cytoplasm > Cytosol. Lysosome Membrane. Translocates to lysosomal membranes in a calcium-dependent fashion.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (91%), Bovine (82%), Horse (82%), Sheep (82%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using PLA2G4F Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using PLA2G4F Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of PLA2G4F using 293 whole cell lysates)
Western Blot (WB)
(Western blot analysis of PLA2G4F using 293 whole cell lysates)
Immunohistochemistry (IHC)
(MBS9609747 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609747 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PLA2G4F antibody
Function: Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine (By similarity).
Similarity: The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).
Similarity: The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 95 kDa
Predicted: 96 kDa
Predicted: 96 kDa
NCBI Official Full Name
cytosolic phospholipase A2 zeta
NCBI Official Synonym Full Names
phospholipase A2 group IVF
NCBI Official Symbol
PLA2G4F
NCBI Official Synonym Symbols
PLA2G4FZ
NCBI Protein Information
cytosolic phospholipase A2 zeta
UniProt Protein Name
Cytosolic phospholipase A2 zeta
Protein Family
UniProt Gene Name
PLA2G4F
UniProt Synonym Gene Names
cPLA2-zeta
Uniprot Description
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine ().
Research Articles on PLA2G4F
Similar Products
Product Notes
The PLA2G4F pla2g4f (Catalog #AAA9609747) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLA2G4F Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G4F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the PLA2G4F pla2g4f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G4F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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