Western Blot (WB)
(All lanes : Anti-PLA2G4F Antibody (Center) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: Raji whole cell lysateLane 3: mouse stomach lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse PLA2G4F Polyclonal Antibody | anti-PLA2G4F antibody
PLA2G4F Antibody (Center)
Western Blot (WB)
(All lanes : Anti-PLA2G4F Antibody (Center) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: Raji whole cell lysateLane 3: mouse stomach lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PLA2G4F Antibody (Center) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: Raji whole cell lysateLane 3: mouse stomach lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
Uniprot Description
PLA2G4F: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine. Has a much higher lysophospholipase and PLA(2) activity than PLA2G4B. Potently inhibited by pyrrolidine-2 and Wyeth-1. Its specific activity, inhibitor sensitivity, and low micromolar calcium dependence are similar to PLA2G4A. Translocates to ruffles and dynamic vesicular structures in a calcium-dependent manner. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ Three isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Phospholipase
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component: cytosol; lysosomal membrane; vesicle
Molecular Function: calcium-dependent phospholipase A2 activity; lysophospholipase activity; metal ion binding; phospholipase A1 activity; phospholipase A2 activity
Biological Process: arachidonic acid secretion; phospholipid catabolic process; phospholipid metabolic process; prostaglandin biosynthetic process
Research Articles on PLA2G4F
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Product Notes
The PLA2G4F pla2g4f (Catalog #AAA9217192) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLA2G4F Antibody (Center) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G4F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the PLA2G4F pla2g4f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLA2G4F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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