Rabbit anti-Human LCAT Polyclonal Antibody | anti-LCAT antibody

LCAT, ID (LCAT, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase) (MaxLight 550)

Cat. Num. AAA6315433

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: LCAT; Polyclonal Antibody; ID (LCAT; Phosphatidylcholine-sterol acyltransferase; Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase) (MaxLight 550); Phospholipid-cholesterol acyltransferase; anti-LCAT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LCAT
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-LCAT antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 285-313 in the Central region of human LCAT, UniProt Accession #P04180.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of K562 cell line lysates (35ug/lane) usingMBS6008402. This demonstrates MBS6008402 dectects the LCAT protien (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6008402 and peroxidase conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6008402 for immunohistochemistry)

Immunofluorescence (IF)

(Confocal Immunofluorescent analysis with 293 cells using MBS6008402 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS6008402 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LCAT antibody

LCAT encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport.

Product Categories/Family for anti-LCAT antibody

Antibodies; Enzymes; Acyltransferase

References

1. Weissglas-Volkov, D., et al. Circ Cardiovasc Genet 3(1):31-38(2010). 2. McGeachie, M., et al. Circulation 120(24):2448-2454(2009). 3. Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009). 4. Voora, D., et al. Circ Cardiovasc Genet 1(2):100-106(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 241429
• NCBI GeneID: 3931
• UniProt Accession #: P04180; Q53XQ3
• Molecular Weight: 49,578 Da
• NCBI Official Full Name: lecithin:cholesterol acyltransferase
• NCBI Official Synonym Full Names: lecithin-cholesterol acyltransferase
• NCBI Official Symbol: LCAT
• NCBI Protein Information: phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• UniProt Protein Name: Phosphatidylcholine-sterol acyltransferase
• UniProt Gene Name: LCAT
• UniProt Entry Name: LCAT_HUMAN

NCBI Description

This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

LCAT: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD); also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure. Defects in LCAT are a cause of fish-eye disease (FED); also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye'). Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Secreted; Lipid Metabolism - glycerophospholipid; EC 2.3.1.43; Transferase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; extracellular region

Molecular Function: apolipoprotein A-I binding; protein binding; phosphatidylcholine-sterol O-acyltransferase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; cholesterol transport; lipoprotein biosynthetic process; phospholipid metabolic process; phosphatidylcholine biosynthetic process; lipoprotein metabolic process

Disease: Lecithin:cholesterol Acyltransferase Deficiency; Fish-eye Disease