Rabbit anti-Human PKN3 Polyclonal Antibody | anti-PKN3 antibody

PKN3 (Serine/Threonine-protein Kinase N3, Protein Kinase PKN-beta, Protein-kinase C-related Kinase 3, PKNBETA, RP11-545E17.1)

Cat. Num. AAA646952

• Gene Names: PKN3; RP11-545E17.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PKN3; Polyclonal Antibody; PKN3 (Serine/Threonine-protein Kinase N3; Protein Kinase PKN-beta; Protein-kinase C-related Kinase 3; PKNBETA; RP11-545E17.1); Anti -PKN3 (Serine/Threonine-protein Kinase N3; anti-PKN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKN3.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MEEGAPRQPGPSQWPPEDEKEVIRRAIQKELKIKEGVENLRRVATDRRHLGHVQQLLRSSNRRLEQLHGELRELHARILLPGPGPGPAEPVASGPRPWAEQLRARHLEALRRQLHVELKVKQGAENMTHTCASGTPKERKLLAAAQQMLRDSQLKVALLRMKISSLEASGSPEPGPELLAEELQHRLHVEAAVAEGAKNVVKLLSSRRTQDRKALAEAQAQLQESSQKLDLLRLALEQLLEQLPPAHPLRSRVTRELRAAVPGYPQPSGTPVKPTALTGTLQVRLLGCEQLLTAVPGRSPAAALASSPSEGWLRTKAKHQRGRGELASEVLAVLKVDNRVVGQTGWGQVAEQSWDQTFVIPLERARELEIGVHWRDWRQLCGVAFLRLEDFLDNACHQLSLSLVPQGLLFAQVTFCDPVIERRPRLQRQERIFSKRRGQDFLRASQMNLGMAAWGRLVMNLLPPCSSPSTISPPKGCPRTPTTLREASDPATPSNFLPKKTPLGEEMTPPPKPPRLYLPQEPTSEETPRTKRPHMEPRTRRGPSPPASPTRKPPRLQDFRCLAVLGRGHFGKVLLVQFKGTGKYYAIKALKKQEVLSRDEIESLYCEKRILEAVGCTGHPFLLSLLACFQTSSHACFVTEFVPGGDLMMQIHEDVFPEPQARFYVACVVLGLQFLHEKKIIYRDLKLDNLLLDAQGFLKIADFGLCKEGIGFGDRTSTFCGTPEFLAPEVLTQEAYTRAVDWWGLGVLLYEMLVGECPFPGDTEEEVFDCIVNMDAPYPGFLSVQGLEFIQKLLQKCPEKRLGAGEQDAEEIKVQPFFRTTNWQALLARTIQPPFVPTLCGPADLRYFEGEFTGLPPALTPPAPHSLLTARQQAAFRDFDFVSERFLEP

• Applicable Applications for anti-PKN3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human PKN3, aa1-889 (NP_037487.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PKN3 expression in transfected 293T cell line by PKN3 polyclonal antibody. Lane 1: PKN3 transfected lysate (99.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PKN3 antibody

PKN3, a protein kinase C-related molecule, mediates malignant cell growth downstream of activated PI3K. PKN3 is required for invasive prostate cell growth as assessed by 3D cell culture assays and in an orthotopic mouse tumor model by inducible expression of short hairpin RNA (shRNA). PKN3 is regulated by PI3K at both the expression level and the catalytic activity level. Therefore, PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of PI3K.

Product Categories/Family for anti-PKN3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 116283712
• NCBI GeneID: 29941
• UniProt Accession #: Q6P5Z2; Q9UM03
• Molecular Weight: 99,421 Da
• NCBI Official Full Name: PKN3 protein
• NCBI Official Synonym Full Names: protein kinase N3
• NCBI Official Symbol: PKN3
• NCBI Official Synonym Symbols: RP11-545E17.1
• NCBI Protein Information: serine/threonine-protein kinase N3; protein kinase PKN-beta; protein-kinase C-related kinase 3
• UniProt Protein Name: Serine/threonine-protein kinase N3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PKN3
• UniProt Synonym Gene Names: PKNBETA
• UniProt Entry Name: PKN3_HUMAN

Uniprot Description

Function: Contributes to invasiveness in malignant prostate cancer. Ref.4

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Two specific sites, Thr-718 (activation loop of the kinase domain) and Thr-860 (turn motif), need to be phosphorylated for its full activation

By similarity.

Subcellular location: Nucleus. Cytoplasm › perinuclear region. Note: Nuclear and perinuclear Golgi region. Ref.1

Tissue specificity: Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues. Ref.1

Domain: The C1 domain does not bind the diacylglycerol (DAG).

Post-translational modification: Autophosphorylated. Ref.1 Ref.4

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.Contains 3 REM (Hr1) repeats.