Western Blot (WB)
(Western Blot analysis in HeLa cell lysate using MBS628129. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human PKN3 Polyclonal Antibody | anti-PKN3 antibody
PKN3, CT (Serine/Threonine-protein Kinase N3, Protein Kinase PKN-beta, Protein-kinase C-related Kinase 3, PKNBETA)
Gene Names
PKN3; RP11-545E17.1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
PKN3; Polyclonal Antibody; CT (Serine/Threonine-protein Kinase N3; Protein Kinase PKN-beta; Protein-kinase C-related Kinase 3; PKNBETA); Anti -PKN3; anti-PKN3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PKN.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-PKN3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:10-1:50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:10-1:50
Immunogen
Synthetic peptide selected from the C-terminal region of human PKN3 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in HeLa cell lysate using MBS628129. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western Blot analysis in HeLa cell lysate using MBS628129. PKNbeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MBS628129, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MBS628129, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Related Product Information for anti-PKN3 antibody
PKN3, a protein kinase C-related molecule, mediates malignant cell growth downstream of activated PI3K. PKN3 is required for invasive prostate cell growth as assessed by 3D cell culture assays and in an orthotopic mouse tumor model by inducible expression of short hairpin RNA (shRNA). PKN3 is regulated by PI3K at both the expression level and the catalytic activity level. Therefore, PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of PI3K.
Product Categories/Family for anti-PKN3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,247 Da
NCBI Official Full Name
PKN3 protein
NCBI Official Synonym Full Names
protein kinase N3
NCBI Official Symbol
PKN3
NCBI Official Synonym Symbols
RP11-545E17.1
NCBI Protein Information
serine/threonine-protein kinase N3; OTTHUMP00000022293; protein kinase PKN-beta; protein-kinase C-related kinase 3
UniProt Protein Name
PKN3 protein
Protein Family
UniProt Gene Name
PKN3
UniProt Entry Name
Q05BU1_HUMAN
Uniprot Description
PKN3: an AGC kinase of the PKN family.
Protein type: EC 2.7.11.13; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKN family
Chromosomal Location of Human Ortholog: 9q34.11
Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; nucleus
Molecular Function: protein binding; protein kinase C activity; ATP binding; protein kinase activity
Biological Process: signal transduction; protein amino acid phosphorylation
Research Articles on PKN3
Similar Products
Product Notes
The PKN3 pkn3 (Catalog #AAA628129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKN3, CT (Serine/Threonine-protein Kinase N3, Protein Kinase PKN-beta, Protein-kinase C-related Kinase 3, PKNBETA) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKN3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the PKN3 pkn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKN3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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