Western Blot (WB)
(Mouse Map4k2 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map4k2 antibody detected the Map4k2 protein (arrow).)
Rabbit anti-Mouse Map4k2 Polyclonal Antibody | anti-Map4k2 antibody
Mouse Map4k2 Antibody (Center)
Western Blot (WB)
(Mouse Map4k2 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map4k2 antibody detected the Map4k2 protein (arrow).)
Western Blot (WB)
(Mouse Map4k2 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map4k2 antibody detected the Map4k2 protein (arrow).)
Ren, M., et al. Proc. Natl. Acad. Sci. U.S.A. 93(10):5151-5155(1996)
NCBI and Uniprot Product Information
Uniprot Description
GCK: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Interacts with TRAF2, TRAF6, MAP3K1/MEKK1 and MAP3K11/MLK3. Interacts with RAB8A. Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined. The tumor necrosis factor (TNF), as well as endotoxins and proinflammatory stimuli such as polyinosine- polycytidine (poly(IC)), lipopolysaccharides (LPS), peptidoglycan (PGN), flagellin, or lipid A activate MAP4K2 by promoting its autophosphorylation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Motility/polarity/chemotaxis; Kinase, protein; EC 2.7.11.1; STE group; STE20 family; KHS subfamily
Cellular Component: Golgi apparatus; membrane; cytoplasm; plasma membrane; intracellular
Molecular Function: transferase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; mitogen-activated protein kinase kinase kinase binding; kinase activity; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: immune system process; vesicle targeting; positive regulation of JNK cascade; response to stress; innate immune response; protein amino acid phosphorylation; phosphorylation; activation of JNK activity
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Product Notes
The Map4k2 map4k2 (Catalog #AAA9204451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 432-459. The Mouse Map4k2 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Map4k2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Map4k2 map4k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Map4k2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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