Rabbit anti-Human PKD2  Polyclonal Antibody | anti-PKD2  antibody

PKD2 (Phospho-S876) polyclonal antibody

Cat. Num. AAA3005009

• Gene Names: PRKD2; PKD2; HSPC187; nPKC-D2
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PKD2 ; Polyclonal Antibody; PKD2 (Phospho-S876) polyclonal antibody; Serine/threonine-protein kinase D2; nPKC-D2; PRKD2; PKD2; HSPC187; anti-PKD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKD2 (phospho-S876) polyclonal antibody detects endogenous levels of PKD2 protein only when phosphorylated at Ser876.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 878

• Applicable Applications for anti-PKD2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human PKD2 around the phosphorylation site of Serine 876
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PKD2  (phospho-S876)  polyclonal antibody at 1:500 dilutionLane1:DLD whole cell lysate (40ug) Lane2:MG63 whole cell lysate (40ug) Lane3:COS-7 whole cell lysate (40ug))

Related Product Information for anti-PKD2  antibody

Protein kinase D2 (PKD2) is one of three members of the protein kinase D family, including PKD1/PKCu and PKD3/PKC?, that belong to the calcium/calmodulin superfamily of serine/threonine protein kinases. PKDs contain a conserved, carboxy-terminal catalytic domain, an amino-terminal regulatory region hallmarked by a PH domain that coordinates subcellular localization, and two zinc-finger/C1 lipid-binding domains that mediate activation of the enzyme in response to diacylglycerol (DAG) or phorbol ester. In addition to lipid-mediated activation, PKD catalytic activity can also be stimulated via phosphorylation of critical serine residues within the activation loop of the enzyme. Novel PKCs, such as PKCeta and PKCepsilon, have been shown to phosphorylate PKD1 at Ser744 and Ser748 (Ser706 and Ser710 in human PKD2), resulting in alleviation of autoinhibition of the enzyme mediated by PH domain interactions with the catalytic domain. Phosphorylation and activation of PKD isoforms has also been described for other upstream kinases. For example, casein kinase 2 (CK2) has been shown to phosphorylate PKD2 at Ser244, which promotes nuclear accumulation of PKD2, phosphorylation of HDAC7, and expression of Nur77. Although only a handfull of PKD2 effectors have been identified, PKD2 has been implicated in regulating an array of cellular events, including cell survival, development, growth, migration, and transformation. PKD2-mediated phosphorylation of at least one known substrate, phosphatidylinositol 4-kinase type IIIbeta (PI4KIIIbeta), also implicates PKD2 in the formation and regulation of exocytotic transport vesicles from the trans Golgi network.

NCBI and Uniprot Product Information

• NCBI GI #: 120659782
• NCBI GeneID: 25865
• NCBI Accession #: NP_001073349.1
• NCBI GenBank Nucleotide #: NP_001073349.1
• UniProt Accession #: Q9BZL6
• Molecular Weight: ~ 105kDa
• NCBI Official Full Name: serine/threonine-protein kinase D2 isoform A
• NCBI Official Synonym Full Names: protein kinase D2
• NCBI Official Symbol: PRKD2
• NCBI Official Synonym Symbols: PKD2; HSPC187; nPKC-D2
• NCBI Protein Information: serine/threonine-protein kinase D2
• UniProt Protein Name: Serine/threonine-protein kinase D2
• UniProt Gene Name: PRKD2
• UniProt Synonym Gene Names: PKD2
• UniProt Entry Name: KPCD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Motility/polarity/chemotaxis; Kinase, protein; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: cell death; positive regulation of cell adhesion; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of angiogenesis; peptidyl-serine phosphorylation; positive regulation of T cell receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell adhesion