Rabbit anti-Human PRKD2 Polyclonal Antibody | anti-PRKD2 antibody

Phospho-PRKD2 (Ser876) Rabbit pAb

Cat. Num. AAA4756856

• Gene Names: PRKD2; PKD2; HSPC187; nPKC-D2
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: PRKD2; Polyclonal Antibody; Phospho-PRKD2 (Ser876) Rabbit pAb; Serine/threonine-protein kinase D2; protein kinase D2; PKD2; HSPC187; nPKC-D2; anti-PRKD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Widely expressed.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-PRKD2 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; IP: 1:20
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser876 of human PKD2
• Conjugation: Unconjugated
• Modification: Phosphoralated
• Cell Position: Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of PKD2 (Phospho-Ser876) in Hela,Hela+TPA lysates using PKD2 (Phospho-Ser876) antibody.Predicted band size:97kDa.Observed band size:105kDa.)

Related Product Information for anti-PRKD2 antibody

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion (PubMed:15604256, PubMed:14743217, PubMed:17077180, PubMed:16928771, PubMed:17962809, PubMed:17951978, PubMed:18262756, PubMed:19192391, PubMed:19001381, PubMed:23503467, PubMed:28428613). May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-717 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B (PubMed:15604256, PubMed:28428613). In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77 (PubMed:17962809). Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation (PubMed:17077180). During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway (PubMed:16928771). During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors (PubMed:19192391). In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane (PubMed:14743217). Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis (PubMed:19001381). In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN (PubMed:18262756). Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells (PubMed:17951978). Plays a regulatory role in angiogenesis and tumor growth by phosphorylating a downstream mediator CIB1 isoform 2, resulting in vascular endothelial growth factor A (VEGFA) secretion (PubMed:23503467).
The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Product Categories/Family for anti-PRKD2 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 120659782
• NCBI GeneID: 25865
• NCBI Accession #: NP_001073349.1
• NCBI GenBank Nucleotide #: NM_001079880.1
• UniProt Accession #: Q9BZL6; Q8TB08; Q9P0T6; Q9Y3X8
• Molecular Weight: 96,750 Da
• NCBI Official Full Name: serine/threonine-protein kinase D2 isoform A
• NCBI Official Synonym Full Names: protein kinase D2
• NCBI Official Symbol: PRKD2
• NCBI Official Synonym Symbols: PKD2; HSPC187; nPKC-D2
• NCBI Protein Information: serine/threonine-protein kinase D2
• UniProt Protein Name: Serine/threonine-protein kinase D2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PRKD2
• UniProt Synonym Gene Names: PKD2
• UniProt Entry Name: KPCD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Motility/polarity/chemotaxis; Kinase, protein; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: cell death; positive regulation of cell adhesion; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of angiogenesis; peptidyl-serine phosphorylation; positive regulation of T cell receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell adhesion