Rabbit anti-Human PJA2 Polyclonal Antibody | anti-PJA2 antibody

Rabbit Polyclonal (IgG) to Human PJA2

Cat. Num. AAA247798

• Gene Names: PJA2; RNF131; Neurodap1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PJA2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human PJA2; Anti-PJA2 Antibody (aa141-190) IHC-plus; KIAA0438; Neurodap1; Praja ring finger 2; Praja2; RING finger protein 131; RNF131; Human PJA2; anti-PJA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PJA2 Antibody detects endogenous levels of total PJA2 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 708

• Applicable Applications for anti-PJA2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human PJA2 (aa141-190).
• Immunogen: PJA2 antibody was raised against synthetic peptide from human PJA2 (aa141-190).
• Antigen Modification: aa141-190
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-PJA2 antibody IHC of human kidney, glomeruli. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from Jurkat cells, using PJA2 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 157412255
• NCBI GeneID: 9867
• NCBI Accession #: NP_055634.3
• NCBI GenBank Nucleotide #: NM_014819.4
• UniProt Accession #: O43164; Q68D49; Q8N1G5; A8K6U4; D3DSZ5
• Molecular Weight: 76,091 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Praja-2
• NCBI Official Synonym Full Names: praja ring finger 2, E3 ubiquitin protein ligase
• NCBI Official Symbol: PJA2
• NCBI Official Synonym Symbols: RNF131; Neurodap1
• NCBI Protein Information: E3 ubiquitin-protein ligase Praja-2; praja2; ring finger protein 131; praja 2, RING-H2 motif containing
• UniProt Protein Name: E3 ubiquitin-protein ligase Praja-2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: PJA2
• UniProt Synonym Gene Names: KIAA0438; RNF131; Praja2
• UniProt Entry Name: PJA2_HUMAN

Uniprot Description

PJA2: Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA- mediated long-term memory processes. Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: Golgi membrane; postsynaptic membrane; endoplasmic reticulum membrane; postsynaptic density; cytoplasm; plasma membrane; cell junction

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: long-term memory; protein ubiquitination