Mouse CDC2 / CDK2 Monoclonal Antibody | anti-CDC2 antibody

Mouse Monoclonal [clone AN4.3] (IgG) to Human CDC2 / CDK2

Cat. Num. AAA245267

• Gene Names: CDK2; CDKN2; p33(CDK2)
• Reactivity: Xenopus, Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein G Purified
• Synonyms: CDC2 / CDK2; Monoclonal Antibody; Mouse Monoclonal [clone AN4.3] (IgG) to Human CDC2 / CDK2; Anti-CDC2 / CDK2 Antibody (clone AN4.3) IHC-plus; CDK2; CDC2; Cell devision kinase 2; Cell division protein kinase 2; CDC2-related protein kinase; Cell division kinase 2; p33 protein kinase; p33(CDK2); CDKN2; Cyclin-dependent kinase 2; Human CDC2; anti-CDC2 antibody

• Host: Mouse
• Reactivity: Xenopus, Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: AN4.3
• Specificity: Recognizes human cdk2, MW 33kD. Does not cross-react with other cdks. Species cross-reactivity: mouse and Xenopus.
• Purity/Purification: Protein G Purified
• Form/Format: 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%
• Concentration: 0.85 mg/ml (varies by lot)
• Sequence Length: 298

• Applicable Applications for anti-CDC2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human cdk2.
• Immunogen Type: Recombinant protein
• Immunogen: CDC2 / CDK2 antibody was raised against recombinant human cdk2.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CDK2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-CDC2 antibody

Cyclin-dependent kinase 2 (CDK2), a CDC2/CDK -type protein kinase, regulates cell-cycle transition from G1 to S phase. CDK2 activation requires formation of a heterodimer with cyclin A, D, or E and phosphorylation by CAK. CDK2 inhibition results from association with p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Two alternatively spliced variants and multiple transcription initiation sites have been reported.

Product Categories/Family for anti-CDC2 antibody

Family: Protein Kinase; Subfamily: CDC2/CDK

NCBI and Uniprot Product Information

• NCBI GI #: 16936528
• NCBI GeneID: 1017
• NCBI Accession #: NP_001789.2
• NCBI GenBank Nucleotide #: NM_001798.4
• UniProt Accession #: P24941; O75100; A8K7C6
• Molecular Weight: 30,035 Da
• NCBI Official Full Name: cyclin-dependent kinase 2 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2
• NCBI Official Symbol: CDK2
• NCBI Official Synonym Symbols: CDKN2; p33(CDK2)
• NCBI Protein Information: cyclin-dependent kinase 2; p33 protein kinase; cdc2-related protein kinase; cell division protein kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cell division protein kinase
• UniProt Gene Name: CDK2
• UniProt Synonym Gene Names: CDKN2
• UniProt Entry Name: CDK2_HUMAN

NCBI Description

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Protein kinase, CMGC; EC 2.7.11.22; Kinase, protein; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; Y chromosome; Cajal body; centrosome; chromosome, telomeric region; transcription factor complex; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; condensed chromosome; nucleus; cytosol; endosome

Molecular Function: protein binding; cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding

Biological Process: G1 DNA damage checkpoint; mitosis; meiosis; histone phosphorylation; positive regulation of transcription, DNA-dependent; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; Ras protein signal transduction; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; DNA replication; centrosome duplication; potassium ion transport; G1/S transition of mitotic cell cycle; positive regulation of DNA replication initiation