Rabbit anti-Mouse CRYM Polyclonal Antibody | anti-CRYM antibody

CRYM, ID (CRYM, THBP, Thiomorpholine-carboxylate dehydrogenase, Mu-crystallin homolog, NADP-regulated thyroid-hormone-binding protein, ketimine reductase) (Azide free) (HRP)

Cat. Num. AAA6289103

• Gene Names: CRYM; THBP; DFNA40
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CRYM; Polyclonal Antibody; ID (CRYM; THBP; Thiomorpholine-carboxylate dehydrogenase; Mu-crystallin homolog; NADP-regulated thyroid-hormone-binding protein; ketimine reductase) (Azide free) (HRP); ketimine reductase; anti-CRYM antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CRYM antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CRYM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the Central region of human CRYM.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CRYM Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the CRYM antibody detected the CRYM protein (arrow).)

Product Categories/Family for anti-CRYM antibody

Antibodies; Crystallins

References

Martins-de-Souza, D., et al. J Psychiatr Res (2010). In press: Al-Kafaji, G., et al. Biochem. Biophys. Res. Commun. 391(4):1585-1591(2010). Malinowska, K., et al. Prostate 69(10):1109-1118(2009). Martins-de-Souza, D., et al. J Psychiatr Res 43(11):978-986(2009). Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503065
• NCBI GeneID: 1428
• NCBI Accession #: NP_001879.1
• NCBI GenBank Nucleotide #: NM_001888.4
• UniProt Accession #: Q14894; Q5HYB7; D5MNX0
• Molecular Weight: 33,776 Da
• NCBI Official Full Name: ketimine reductase mu-crystallin
• NCBI Official Synonym Full Names: crystallin, mu
• NCBI Official Symbol: CRYM
• NCBI Official Synonym Symbols: THBP; DFNA40
• NCBI Protein Information: ketimine reductase mu-crystallin; mu-crystallin homolog; thiomorpholine-carboxylate dehydrogenase; NADP-regulated thyroid-hormone binding protein
• UniProt Protein Name: Ketimine reductase mu-crystallin
• Protein Family: Ketimine reductase
• UniProt Gene Name: CRYM
• UniProt Synonym Gene Names: THBP
• UniProt Entry Name: CRYM_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]

Uniprot Description

CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.

Protein type: Lyase; EC 1.5.1.25

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity

Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process

Disease: Deafness, Autosomal Dominant 40