Rabbit anti-Mouse CTLA-4 Polyclonal Antibody | anti-CTLA4 antibody

Anti-CTLA-4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111673

• Gene Names: Ctla4; Cd152; Ly-56; Ctla-4
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CTLA-4; Polyclonal Antibody; Anti-CTLA-4 Antibody; Rabbit Polyclonal; CTLA-4/CD152 Antibody; Rabbit PAb; Antigen Affinity Purified; cytotoxic T-lymphocyte-associated protein 4; Anti-Cd152 Antibody; Anti-Ctla-4 Antibody; Anti-Ly-56 Antibody; anti-CTLA4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CTLA-4/CD152
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTLA4 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 1-2uL/mg of lysate
• Immunogen: Recombinant Mouse CTLA-4/CD152 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CTLA-4/CD152 (rM CTLA-4/CD152). CTLA-4/CD152 specific IgG was purified by Mouse CTLA-4/CD152 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(Mouse CTLA4 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-Mouse CTLA4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CTLA4 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)

Western Blot (WB)

(Anti-CTLA4 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDaObserved band size:26 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CTLA4 antibody

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

Product Categories/Family for anti-CTLA4 antibody

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.

NCBI and Uniprot Product Information

• NCBI GI #: 31981847
• NCBI GeneID: 12477
• NCBI Accession #: NP_033973.2
• NCBI GenBank Nucleotide #: NM_009843.4
• UniProt Accession #: P09793; Q9QZZ7
• Molecular Weight: 24,993 Da
• NCBI Official Full Name: cytotoxic T-lymphocyte protein 4 isoform 1
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte-associated protein 4
• NCBI Official Symbol: Ctla4
• NCBI Official Synonym Symbols: Cd152; Ly-56; Ctla-4
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• Protein Family: Cytotoxic T-lymphocyte protein
• UniProt Gene Name: Ctla4
• UniProt Synonym Gene Names: Cd152; CTLA-4
• UniProt Entry Name: CTLA4_MOUSE

NCBI Description

This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; clathrin-coated endocytic vesicle; external side of plasma membrane

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of apoptosis; immune system process; negative regulation of regulatory T cell differentiation; negative regulation of immune response; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus