Rabbit ATG4A Polyclonal Antibody | anti-ATG4A antibody

ATG4A (Autophagy Related Protein 4 Homolog A, APG4A, AUTL2) (Biotin)

Cat. Num. AAA6407545

• Gene Names: ATG4A; APG4A; AUTL2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ATG4A; Polyclonal Antibody; ATG4A (Autophagy Related Protein 4 Homolog A; APG4A; AUTL2) (Biotin); anti-ATG4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ATG4A. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ATG4A antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; IP: 1:20-1:100; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human ATG4A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATG4A antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human lung cancer using ATG4A antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded rat brain using ATG4A antibody at dilution of 1:200 (400x lens).)

Testing Data

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Product Categories/Family for anti-ATG4A antibody

Antibodies; Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 30795252
• NCBI GeneID: 115201
• NCBI Accession #: NP_443168.2
• NCBI GenBank Nucleotide #: NM_052936.3
• UniProt Accession #: Q8WYN0; O95534; Q5JYY9; Q5JYZ0; Q86VE5; Q96KQ0; Q96KQ1; A6NCH2; B2RAZ7; D3DUY0
• Molecular Weight: 45,378 Da
• NCBI Official Full Name: cysteine protease ATG4A isoform a
• NCBI Official Synonym Full Names: autophagy related 4A, cysteine peptidase
• NCBI Official Symbol: ATG4A
• NCBI Official Synonym Symbols: APG4A; AUTL2
• NCBI Protein Information: cysteine protease ATG4A; hAPG4A; autophagin 2; autophagin-2; APG4 autophagy 4 homolog A; AUT-like 2 cysteine endopeptidase; ATG4 autophagy related 4 homolog A; AUT-like 2, cysteine endopeptidase; autophagy-related protein 4 homolog A; autophagy-related cy
• UniProt Protein Name: Cysteine protease ATG4A
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4A
• UniProt Synonym Gene Names: APG4A; AUTL2; hAPG4A
• UniProt Entry Name: ATG4A_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Microtubule-binding; Autophagy; Protease

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; C-terminal protein lipidation; protein processing; protein targeting to membrane; proteolysis; autophagic vacuole formation; cellular response to nitrogen starvation