Typical Testing Data/Standard Curve (for reference only)
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General Neutral Protease Assay Kit | npr assay kit
Neutral Protease Microplate Assay Kit
Reactivity
General
Applications
Functional Assay
Synonyms
Neutral Protease; Neutral Protease Microplate Assay Kit; npr assay kit
Reactivity
General
Sequence Length
566
Applicable Applications for npr assay kit
Functional Assay
Samples
Urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples
Detection Range
0.05 mmol/L - 5 mmol/L
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for npr assay kit
Background/Introduction: Neutral Protease is frequently used as a secondary enzyme in conjunction with collagenase and/or other proteases in many primary cell isolation and tissue dissociation applications. Neutral Protease dissociates fibroblast-like cells more efficiently than epithelial-like cells so it has also been used for differential isolation and culture applications. Other advantages are its non-mammalian (bacterial) source and its ability to be inhibited by EDTA. The assay is initiated with the enzymatic catalysis of casein by NP in neutral environment. The enzyme catalysed reaction products can be measured at a colorimetric readout at 660 nm.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
60,919 Da
NCBI Official Full Name
neutral protease
UniProt Protein Name
Bacillolysin
Protein Family
UniProt Gene Name
npr
UniProt Synonym Gene Names
nprC
UniProt Entry Name
NPRE_BACCE
Uniprot Description
Extracellular zinc metalloprotease.
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Product Notes
The General Neutral Protease npr (Catalog #AAA8243192) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8243192 Assay Kit recognizes General Neutral Protease. AAA Biotech's Neutral Protease can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the Neutral Protease npr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neutral Protease, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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