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Western Blot (WB) (PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).)

Rabbit anti-Human, Mouse PIGX Polyclonal Antibody | anti-Pigx antibody

PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein)

Gene Names
Pigx; PIG-X; 2010319C14Rik
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
PIGX; Polyclonal Antibody; NT (PIGX; Phosphatidylinositol-glycan biosynthesis class X protein); Anti -PIGX; anti-Pigx antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Pigx antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunogen
PIGX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the N-terminal region of human PIGX.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).)

Western Blot (WB) (PIGX Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PIGX antibody detected the PIGX protein (arrow).)

Immunohistochemistry (IHC)

(PIGX antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGX antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (PIGX antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PIGX antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Pigx antibody
Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM (By similarity).
Product Categories/Family for anti-Pigx antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,644 Da
NCBI Official Full Name
phosphatidylinositol-glycan biosynthesis class X protein isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis, class X
NCBI Official Symbol
Pigx
NCBI Official Synonym Symbols
PIG-X; 2010319C14Rik
NCBI Protein Information
phosphatidylinositol-glycan biosynthesis class X protein; phosphatidylinositol glycan, class X
UniProt Protein Name
Phosphatidylinositol-glycan biosynthesis class X protein
UniProt Gene Name
Pigx
UniProt Synonym Gene Names
PIG-X
UniProt Entry Name
PIGX_MOUSE

NCBI Description

This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM

By similarity.

Pathway: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subunit structure: Interacts with PIGM

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein

By similarity.

Post-translational modification: N-glycosylated

By similarity.

Sequence similarities: Belongs to the PIGX family.

Sequence caution: The sequence BAC25223.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.

Research Articles on Pigx

Similar Products

Product Notes

The Pigx pigx (Catalog #AAA648875) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIGX, NT (PIGX, Phosphatidylinositol-glycan biosynthesis class X protein) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIGX can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the Pigx pigx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIGX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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