Rabbit anti-Human PIGO Polyclonal Antibody | anti-PIGO antibody

PIGO, CT (PIGO, GPI ethanolamine phosphate transferase 3, Phosphatidylinositol-glycan biosynthesis class O protein) (FITC)

Cat. Num. AAA6333874

• Gene Names: PIGO; HPMRS2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIGO; Polyclonal Antibody; CT (PIGO; GPI ethanolamine phosphate transferase 3; Phosphatidylinositol-glycan biosynthesis class O protein) (FITC); Phosphatidylinositol-glycan biosynthesis class O protein; anti-PIGO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 1089

• Applicable Applications for anti-PIGO antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PIGO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 954-983 amino acids from the C-terminal region of human PIGO.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PIGO Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the PIGO antibody detected the PIGO protein (arrow).)

Related Product Information for anti-PIGO antibody

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least two alternatively spliced transcripts encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-PIGO antibody

Antibodies; Enzymes; Glycosyltransferase

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Humphray, S.J., et al. Nature 429(6990):369-374(2004). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 23397648
• NCBI GeneID: 84720
• NCBI Accession #: NP_116023.2; NP_690577.2
• NCBI GenBank Nucleotide #: NP_116023.2
• UniProt Accession #: Q8TEQ8
• NCBI Official Full Name: GPI ethanolamine phosphate transferase 3 isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis class O
• NCBI Official Symbol: PIGO
• NCBI Official Synonym Symbols: HPMRS2
• NCBI Protein Information: GPI ethanolamine phosphate transferase 3
• UniProt Protein Name: GPI ethanolamine phosphate transferase 3
• UniProt Gene Name: PIGO
• UniProt Synonym Gene Names: PIG-O
• UniProt Entry Name: PIGO_HUMAN

NCBI Description

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]