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Western Blot (WB) (Western blot analysis of PIGN Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). PIGN (arrow) was detected using the purified Pab.)

Rabbit anti-Human PIGN Polyclonal Antibody | anti-Pign antibody

PIGN, NT (PIGN, MCD4, GPI ethanolamine phosphate transferase 1, MCD4 homolog, Phosphatidylinositol-glycan biosynthesis class N protein)

Gene Names
Pign; PIG-N; Gm20308
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
PIGN; Polyclonal Antibody; NT (PIGN; MCD4; GPI ethanolamine phosphate transferase 1; MCD4 homolog; Phosphatidylinositol-glycan biosynthesis class N protein); Anti -PIGN; anti-Pign antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Pign antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
PIGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213~243 amino acids from the N-terminal region of human PIGN.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIGN Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). PIGN (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of PIGN Antibody (N-term) in mouse NIH-3T3 cell line lysates (35ug/lane). PIGN (arrow) was detected using the purified Pab.)
Related Product Information for anti-Pign antibody
PIGN is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor.
Product Categories/Family for anti-Pign antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
105,045 Da
NCBI Official Full Name
Pign protein
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis, class N
NCBI Official Symbol
Pign
NCBI Official Synonym Symbols
PIG-N; Gm20308
NCBI Protein Information
GPI ethanolamine phosphate transferase 1; phosphatidylinositol glycan, class N; phosphatidylinositol-glycan biosynthesis class N protein
UniProt Protein Name
GPI ethanolamine phosphate transferase 1
UniProt Gene Name
Pign
UniProt Synonym Gene Names
PIG-N
UniProt Entry Name
PIGN_MOUSE

Uniprot Description

PIGN: Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor. Defects in PIGN are the cause of multiple congenital anomalies-hypotonia-seizures syndrome type 1 (MCAHS1). An autosomal recessive disorder characterized by neonatal hypotonia, lack of psychomotor development, seizures, dysmorphic features, and variable congenital anomalies involving the cardiac, urinary, and gastrointestinal systems. Most affected individuals die before 3 years of age. Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.

Protein type: Transferase; Endoplasmic reticulum; Membrane protein, multi-pass; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; EC 2.-.-.-

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: transferase activity; Kdo transferase activity; catalytic activity; phosphotransferase activity, for other substituted phosphate groups

Biological Process: metabolic process; GPI anchor biosynthetic process

Similar Products

Product Notes

The Pign pign (Catalog #AAA6002654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIGN, NT (PIGN, MCD4, GPI ethanolamine phosphate transferase 1, MCD4 homolog, Phosphatidylinositol-glycan biosynthesis class N protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIGN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Pign pign for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIGN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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