Human Neuropeptide Y Receptor Y1 ELISA Kit | NPYR1 elisa kit

Human Neuropeptide Y Receptor Y1 ELISA Kit

Cat. Num. AAA761717

• Gene Names: NPY1R; NPYR; NPY1-R
• Reactivity: Human
• Synonyms: Neuropeptide Y Receptor Y1; Human Neuropeptide Y Receptor Y1 ELISA Kit; NPYR1; NPYR1 elisa kit

• Reactivity: Human
• Sequence Length: 384

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NPYR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NPYR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NPYR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NPYR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NPYR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 189285
• NCBI GeneID: 4886
• Molecular Weight: 44,392 Da
• NCBI Official Full Name: neuropeptide Y receptor Y1
• NCBI Official Synonym Full Names: neuropeptide Y receptor Y1
• NCBI Official Symbol: NPY1R
• NCBI Official Synonym Symbols: NPYR; NPY1-R
• NCBI Protein Information: neuropeptide Y receptor type 1
• UniProt Protein Name: Neuropeptide Y receptor type 1
• UniProt Gene Name: NPY1R
• UniProt Synonym Gene Names: NPYR; NPYY1; NPY1-R
• UniProt Entry Name: NPY1R_HUMAN

NCBI Description

This gene belongs to the G-protein-coupled receptor superfamily. The encoded transmembrane protein mediates the function of neuropeptide Y (NPY), a neurotransmitter, and peptide YY (PYY), a gastrointestinal hormone. The encoded receptor undergoes fast agonist-induced internalization through clathrin-coated pits and is subsequently recycled back to the cell membrane. Activation of Y1 receptors may result in mobilization of intracellular calcium and inhibition of adenylate cyclase activity. [provided by RefSeq, Aug 2013]

Uniprot Description

NPY1R: Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is NPY > [Pro-34] PYY, PYY and [Leu-31, Pro-34] NPY > NPY (2-36) > [Ile-31, Gln-34] PP and PYY (3-36) > PP > NPY free acid. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4q31.3-q32

Cellular Component: axon; cytosol; integral to plasma membrane; plasma membrane; synaptic vesicle

Molecular Function: neuropeptide Y receptor activity; pancreatic polypeptide receptor activity; peptide YY receptor activity; protein binding

Biological Process: blood circulation; cell surface receptor linked signal transduction; feeding behavior; G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; glucose metabolic process; locomotory behavior; negative regulation of hormone secretion; neuropeptide signaling pathway; peristalsis; positive regulation of vasoconstriction; reduction of cytosolic calcium ion concentration; regulation of blood pressure; regulation of multicellular organism growth; response to estradiol stimulus; sensory perception of pain