Rabbit PICK1 (PRKCABP) Polyclonal Antibody | anti-PICK1 antibody

PICK1 (PRKCABP) Antibody (N-term)

Cat. Num. AAA9201820

• Gene Names: PICK1; PICK; PRKCABP
• Reactivity: Human, mouse (Predicted Reactivity: Monkey, Rat)
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PICK1 (PRKCABP); Polyclonal Antibody; PICK1 (PRKCABP) Antibody (N-term); PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein; PICK1; PRKCABP; anti-PICK1 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Monkey, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PICK1 (PRKCABP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PICK1 (PRKCABP).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 415

• Applicable Applications for anti-PICK1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-PRKCABP Pab is used in Western blot to detect PRKCABP in Y79 cell lysate.)

Related Product Information for anti-PICK1 antibody

PDZ domain, but not the AH domain, of PICK1 interacts with the C termini of the GTP-bound forms of ADP-ribosylation factor-1 (ARF1) and ARF3. The interactions with ARF5 and ARF6 are weak, suggesting that the PICK1 interaction is specific for class I ARFs and that it may regulate Golgi-to-endoplasmic reticulum vesicle transport. The PDZ domain of rat Pick1 interacts with the last 10 amino acids of the short C-terminal alternative splice variants of AMPA receptor subunits. It has thus been proposed that E-S-V/I-K-I, a sequence found in these 10 amino acids, is a novel PDZ-binding motif. PRKCA phosphorylates Pick1 efficiently but binds Pick1 in both the phosphorylated and unphosphorylated states. Consistent with a neuronal role for PICK1, the mouse homolog interacts with mouse AMPA glutamate receptors and colocalizes at excitatory synapses in the brain. Metabotropic glutamate receptor-7 (mGluR7) localizes specifically to presynaptic active zones. The extreme C-terminal 3 amino acids of mGluR7 have been shown to interact with the PDZ domain of PICK1. Immunofluorescence microscopy demonstrated that both proteins are localized at excitatory synapses in hippocampal neurons, with clustering of mGluR7 at synapses requires PICK1 C-terminal PDZ-binding residues. Mutant mGluR7 lacking the PDZ-binding residues localized diffusely along axons rather than at the synapse, suggesting a role for Pick1 as a scaffolding molecule at presynaptic sites.

Product Categories/Family for anti-PICK1 antibody

Neuroscience; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 88758600
• NCBI GeneID: 9463
• NCBI Accession #: NP_001034672.1; NP_001034673.1; NP_036539.1
• NCBI GenBank Nucleotide #: NM_001039583.1
• UniProt Accession #: Q9NRD5; O95906; B3KS52
• Molecular Weight: 46600
• NCBI Official Full Name: PRKCA-binding protein
• NCBI Official Synonym Full Names: protein interacting with PRKCA 1
• NCBI Official Symbol: PICK1
• NCBI Official Synonym Symbols: PICK; PRKCABP
• NCBI Protein Information: PRKCA-binding protein
• UniProt Protein Name: PRKCA-binding protein
• Protein Family: PRKCA-binding protein
• UniProt Gene Name: PICK1
• UniProt Synonym Gene Names: PRKCABP
• UniProt Entry Name: PICK1_HUMAN

NCBI Description

The protein encoded by this gene contains a PDZ domain, through which it interacts with protein kinase C, alpha (PRKCA). This protein may function as an adaptor that binds to and organizes the subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, as well as non-voltage gated sodium channels, and may target PRKCA to these membrane proteins and thus regulate their distribution and function. This protein has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PICK1: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ASIC1/ASIC3 channel.

Protein type: Cell development/differentiation; Adaptor/scaffold; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: presynaptic membrane; Golgi apparatus; postsynaptic membrane; neuron projection; cytoskeleton; mitochondrion; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: actin filament binding; protein C-terminus binding; identical protein binding; protein domain specific binding; protein binding; enzyme binding; protein kinase C binding; G-protein-coupled receptor binding; metal ion binding; ATPase activity; receptor binding

Biological Process: glial cell development; DNA methylation during gametogenesis; positive regulation of receptor internalization; monoamine transport; receptor clustering; protein amino acid phosphorylation; cellular response to glucose starvation; synaptic transmission; protein kinase C activation; retrograde vesicle-mediated transport, Golgi to ER; DNA methylation during embryonic development; neuronal ion channel clustering; protein targeting