Rabbit anti-Human NCS1 Polyclonal Antibody | anti-NCS1 antibody

NCS1, ID (Neuronal Calcium Sensor 1, NCS-1, Frequenin Homolog, Frequenin-like Protein, Frequenin-like Ubiquitous Protein, FLUP, FREQ) (Biotin)

Cat. Num. AAA6476898

• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NCS1; Polyclonal Antibody; ID (Neuronal Calcium Sensor 1; NCS-1; Frequenin Homolog; Frequenin-like Protein; Frequenin-like Ubiquitous Protein; FLUP; FREQ) (Biotin); anti-NCS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NCS1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NCS1 antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; WB: 1:50-1:100; FC/FACS: 1:10-1:50; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human NCS1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NCS1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NCS1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ZR-75-1 cells using NCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-NCS1 antibody

Antibodies; Neuroscience

References

1. Koh, P.O., et al., Proc. Natl. Acad. Sci. USA 100(1):313-317 (2003). 2. Bourne, Y., et al., J. Biol. Chem. 276(15):11949-11955 (2001). 3. Burgoyne, R.D., et al., Biochem. J. 353(Pt 1), 1-12 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 548340
• UniProt Accession #: P36610
• Molecular Weight: 21.747
• Protein Family: Purine-uracil permease