Rabbit PICK1 Polyclonal Antibody | anti-PICK1 antibody

PICK1 Antibody

Cat. Num. AAA9408250

• Gene Names: PICK1; PICK; PRKCABP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PICK1; Polyclonal Antibody; PICK1 Antibody; MGC15204; PICK; PRKCABP; anti-PICK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PICK1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 415

• Applicable Applications for anti-PICK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human PICK1.
• Target Name: PICK1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PICK1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human rectum using PICK1 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using PICK1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PICK1 antibody

Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKC activation. PKC , which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKC . PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.

Product Categories/Family for anti-PICK1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 88758600
• NCBI GeneID: 9463
• NCBI Accession #: NP_001034672.1
• NCBI GenBank Nucleotide #: NM_001039583.1
• UniProt Accession #: Q9NRD5; O95906; B3KS52
• Molecular Weight: 39,808 Da
• NCBI Official Full Name: PRKCA-binding protein
• NCBI Official Synonym Full Names: protein interacting with PRKCA 1
• NCBI Official Symbol: PICK1
• NCBI Official Synonym Symbols: PICK; PRKCABP
• NCBI Protein Information: PRKCA-binding protein
• UniProt Protein Name: PRKCA-binding protein
• Protein Family: PRKCA-binding protein
• UniProt Gene Name: PICK1
• UniProt Synonym Gene Names: PRKCABP
• UniProt Entry Name: PICK1_HUMAN

NCBI Description

The protein encoded by this gene contains a PDZ domain, through which it interacts with protein kinase C, alpha (PRKCA). This protein may function as an adaptor that binds to and organizes the subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, as well as non-voltage gated sodium channels, and may target PRKCA to these membrane proteins and thus regulate their distribution and function. This protein has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PICK1: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ASIC1/ASIC3 channel.

Protein type: Ligand, receptor tyrosine kinase; Adaptor/scaffold; Cell development/differentiation

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: presynaptic membrane; Golgi apparatus; postsynaptic membrane; neuron projection; cytoskeleton; mitochondrion; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: protein C-terminus binding; actin filament binding; identical protein binding; protein domain specific binding; protein binding; enzyme binding; protein kinase C binding; G-protein-coupled receptor binding; metal ion binding; ATPase activity; receptor binding

Biological Process: glial cell development; synaptic transmission; cellular response to glucose starvation; DNA methylation during gametogenesis; positive regulation of receptor internalization; monoamine transport; protein kinase C activation; retrograde vesicle-mediated transport, Golgi to ER; neuronal ion channel clustering; DNA methylation during embryonic development; receptor clustering; protein amino acid phosphorylation; protein targeting