Rabbit Piccolo Polyclonal Antibody | anti-PCLO antibody

Piccolo Antibody

Cat. Num. AAA806832

• Gene Names: PCLO; ACZ
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Piccolo; Polyclonal Antibody; Piccolo Antibody; ACZ; Aczonin; Brain-derived HLMN protein; DKFZp779G1236; KIAA0559; Multidomain presynaptic cytomatrix protein; PCLO; PCLO_HUMAN; Piccolo (presynaptic cytomatrix protein); Piccolo mouse homolog of Pico; Presynaptic cytomatrix protein; Protein piccolo; anti-PCLO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 4935

• Applicable Applications for anti-PCLO antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1:1000 (WB)
• Certificate of Analysis: 1ug/mL of SPC-197 was sufficient for detection of Piccolo in 10ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
• Conjugate: Unconjugated
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.)

Western Blot (WB)

(Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (SPC-197) at 1:1000.)

Related Product Information for anti-PCLO antibody

Background Info: Detects ~550kDa corresponding to the molecular weight of Piccolo. Multiple isoforms can be detected. No cross-reactivity to Bassoon.

Scientific Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Product Categories/Family for anti-PCLO antibody

Neuroscience

References

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

NCBI and Uniprot Product Information

• NCBI GI #: 150170670
• NCBI GeneID: 27445
• NCBI Accession #: NP_055325.2
• NCBI GenBank Nucleotide #: NM_014510.2
• UniProt Accession #: Q9Y6VO; O43373; O60305; Q08E72; Q9BVC8; Q9UIV2; Q9Y6U9; A4D1A7; A6NNX9; Q9Y6V0
• Molecular Weight: 537,886 Da
• NCBI Official Full Name: protein piccolo isoform 2
• NCBI Official Synonym Full Names: piccolo presynaptic cytomatrix protein
• NCBI Official Symbol: PCLO
• NCBI Official Synonym Symbols: ACZ
• NCBI Protein Information: protein piccolo; aczonin; piccolo (presynaptic cytomatrix protein)
• UniProt Protein Name: Protein piccolo
• Protein Family: Protein piccolo
• UniProt Gene Name: PCLO
• UniProt Synonym Gene Names: ACZ; KIAA0559
• UniProt Entry Name: PCLO_HUMAN

NCBI Description

The protein encoded by this gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

piccolo: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: synaptic vesicle; cytoskeleton; membrane; postsynaptic density; synapse; cell junction

Molecular Function: calcium-dependent phospholipid binding; transporter activity; calcium ion binding; profilin binding

Biological Process: regulation of exocytosis; synaptic vesicle exocytosis; cAMP-mediated signaling; insulin secretion; cytoskeleton organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 3