Mouse anti-Human, Rat Piccolo Monoclonal Antibody | anti-Pclo antibody

Piccolo Antibody: ATTO 488

Cat. Num. AAA800704

• Gene Names: Pclo; Acz; Pico
• Reactivity: Human, Rat
• Applications: Western Blot, ELISA
• Synonyms: Piccolo; Monoclonal Antibody; Piccolo Antibody: ATTO 488; ACZ; Aczonin; PCLO; Pico; presynaptic cytomatrix protein antibody; anti-Pclo antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: 6H9-B6
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 4863

• Applicable Applications for anti-Pclo antibody: Western Blot (WB), ELISA (EIA)
• Conjugate: ATTO 488
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western Blot analysis of Rat and mouse brain lysates showing detection of Piccolo protein using Mouse Anti-Piccolo Monoclonal Antibody, Clone 6H9-B6. Primary Antibody: Mouse Anti-Piccolo Monoclonal Antibody at 1:1000. (1) Mouse brain lysate; (2) Rat brain lysate.)

Related Product Information for anti-Pclo antibody

Background Info: No cross-reactivity to Bassoon, band at ~550kDa correspondng to the molecular weight of Piccolo.

Scientific Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Product Categories/Family for anti-Pclo antibody

Neuroscience

References

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038- 6047.

NCBI and Uniprot Product Information

• NCBI GI #: 160707978
• NCBI GeneID: 26875
• NCBI Accession #: NP_001104266.1
• NCBI GenBank Nucleotide #: NM_001110796.2
• UniProt Accession #: Q9QYX7; Q8CF91; Q8CF92; Q9QYX6; Q9QZJ0; E9QK94
• Molecular Weight: 528,257 Da
• NCBI Official Full Name: protein piccolo isoform 2
• NCBI Official Synonym Full Names: piccolo (presynaptic cytomatrix protein)
• NCBI Official Symbol: Pclo
• NCBI Official Synonym Symbols: Acz; Pico
• NCBI Protein Information: protein piccolo; aczonin; brain-derived HLMN protein; multidomain presynaptic cytomatrix protein
• UniProt Protein Name: Protein piccolo
• Protein Family: Protein piccolo
• UniProt Gene Name: Pclo
• UniProt Synonym Gene Names: Acz
• UniProt Entry Name: PCLO_MOUSE

Uniprot Description

piccolo: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: cell; postsynaptic density; synapse; intracellular; cell junction

Molecular Function: protein binding; calcium-dependent phospholipid binding; metal ion binding; calcium ion binding; profilin binding

Biological Process: regulation of exocytosis; cAMP-mediated signaling; insulin secretion; cytoskeleton organization and biogenesis; synaptic vesicle targeting