Western Blot (WB)
(WB: SKMEL-19 cell lysate was probed with MBS600758 (1:20,000 dilution).)
Mouse anti-Human NY-ESO-1 Monoclonal Antibody | anti-CTAG1A antibody
NY-ESO-1
Reactivity
Human
Applications
Western Blot
Purity
AscitesAscites
Synonyms
NY-ESO-1; Monoclonal Antibody; Anti -NY-ESO-1; anti-CTAG1A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
4H362
Specificity
Recognizes human NY-ESO-1 protein, Mr 22kD.
Purity/Purification
Ascites
Ascites
Ascites
Form/Format
Supplied as a liquid, 0.05% sodium azide
Applicable Applications for anti-CTAG1A antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:5000-1:20,000 detects NY-ESO-1 protein in RIPA lysates from SKMEL-19 cells. SKMEL-19 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N8590 (1:20,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilution to be determined by the researcher.
Dilution: Western Blot: 1:5000-1:20,000 detects NY-ESO-1 protein in RIPA lysates from SKMEL-19 cells. SKMEL-19 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N8590 (1:20,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilution to be determined by the researcher.
Immunogen
NY-ESO-1 full-length recombinant protein
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WB: SKMEL-19 cell lysate was probed with MBS600758 (1:20,000 dilution).)
Western Blot (WB)
(WB: SKMEL-19 cell lysate was probed with MBS600758 (1:20,000 dilution).)
Related Product Information for anti-CTAG1A antibody
Human gene NY-ESO-1 codes for antigens which are expressed in malignancies of various histological types but not in normal tissues except testis.
Product Categories/Family for anti-CTAG1A antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
17,992 Da
NCBI Official Full Name
NY-ESO-1 protein
UniProt Protein Name
Cancer/testis antigen 1
Protein Family
UniProt Gene Name
CTAG1A
UniProt Synonym Gene Names
CTAG; CTAG1; ESO1; LAGE2; LAGE2A
UniProt Entry Name
CTG1B_HUMAN
Uniprot Description
CTAG1B: is an antigen that is overexpressed in many cancers but that is also expressed in normal testis. This gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence. [provided by RefSeq, Jan 2012]
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cytoplasm
Molecular Function: protein binding
Similar Products
Product Notes
The NY-ESO-1 ctag1a (Catalog #AAA600758) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NY-ESO-1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NY-ESO-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:5000-1:20,000 detects NY-ESO-1 protein in RIPA lysates from SKMEL-19 cells. SKMEL-19 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N8590 (1:20,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the NY-ESO-1 ctag1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NY-ESO-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
